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RE Resource, Chapter 1
For example, Sal I has three sites in the smaller molecule of Ad-2 and two sites ... single site in the multiple cloning region of one of the pGEM® Vectors(a). ... A number of Promega's restriction enzymes are useful in chromosomal mapping ... contaminants are easily eliminated before the final product is prepared. ...
http://www.promega.com/guides/re_guide/chapone/1_6.htm...
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NEMO antibody, NFkappaB essential modulator Antibody, Rabbit ...
[ 4504631 NP_003630.1 Q7LBY6 ]. ( GenWay 18-001-30027 online datasheet ) ... Storage: 2 degree C to 8 degree C for 3 months and at -20 degree C for long term . ... Cells expressing the mutated X chromosome are eliminated selectively around ..... Somatostatin receptor type 5, SOX9 (SRY (sex determining region Y)- box. ...
http://www.genwaybio.com/product_info.php?products_id=30027...
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USER GUIDE
to insert efficiently into virtually any desired DNA target.1,2,3,4,5,6 ... mutated to re-target insertion into a user-specified chromosomal gene. .... From the output design options, locate the region of the gene you want to ..... compensate for gene loss, e.g., introduce a metabolite eliminated by the knockout. ...
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_...
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Focus 21.2
A. The most common fusing agent is polyethylene glycol. A molecular weight range of 4000–6000 .... mentation was eliminated and hybridoma .... cytogeneticist for chromosome number,. F O C U S ( 1 9 9 9 ) V O L U M E 2 1 ...... locus containing a GCC expansion region. 50-µl reactions contained ...
http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf/fo...
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Electrophoresis Gel Stains
eliminated with Imperial™ Protein Stain. In addition to faster protein band ... 3 x 1 L. Sufficient reagent to stain up to 150 mini gels (8 cm x 10 cm). .... gel-staining irregularities common to the method, including high .... Disulfide connectivity of recombinant C-terminal region of human thrombospondin. ...
http://www.piercenet.com/files/ElectroGelStains.pdf...
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Bioluminescence Reporters Protocols and Applications Guide
Although most people are aware of bioluminescence primarily through the nighttime ... reporter gene of interest into the chromosome can be selected and propagated when a ... improvements in assay chemistry have nearly eliminated this problem. ... and ii) we applied random mutagenesis to the C-terminal region and ...
http://www.promega.com/paguide/chap8.htm...
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10.4.pdf
into its chromosome 2(1 copies oí the bacteriophage mu genome. 1 was also involved in several experiments on the .... TnA transposed into a nonessential region of pMB9. The experi- ... one of the three Pst 1 sites present in TnA was located in the .... contamination is eliminated by protein expression in eukaryotic ...
http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf/fo...
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Bioluminescence Reporters Protocols and Applications Guide
1. B. Bioluminescence Reporters. 2. II. Luciferase Genes and Vectors. 3 ..... interest into the chromosome can be selected and .... have nearly eliminated this problem. In addition, the ... the C-terminal region and selected brightly luminescent ..... Easy transfer from one vector to another: Common ...
https://www.promega.com/paguide/chap8.pdf...
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Expressions 6.5
variation due to chromosomal position effects. ..... are eliminated with the stable, plasmid-based. InsectSelect™ System. ... This promoter is active in many common insect cell types including Sf9, Sf21, ... include the enhancer-promoter region as well as the ..... 10-fold increase in the number of colonies and 1/3 ...
http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf/ex...
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