DAVIS SEQUENCING, INC.

We offer DNA sequencing of single stranded or double stranded DNA samples from purified plasmids and PCR products. Our sequencing reactions are performed using the Applied Biosystems Big Dye Terminator V3.0 sequencing chemistry. One sequencing reaction consists of one DNA template (customer supplied plasmid DNA or PCR product) being sequenced with one primer (customer supplied primer or one of our supplied primers). We supply several frequently used sequencing primers for free, but the template DNA samples are supplied by our customers.
Our sequencing reaction read-lengths are normally between 800 and 900 bases (with a basecalling accuracy >99%) when run on our ABI 3730 DNA sequencers. Sequencing read-lengths are dependent upon several factors including template DNA concentration, purity and base composition (poly A and/or T regions, GC content and short tandem repeats) as well as primer binding specificity.

Since DNA sample purity and concentration are the two most critical factors to the success of automated sequencing reactions, we highly recommend that customers use the QIAGEN® plasmid DNA prep kits to purify their plasmid DNA samples. For purifying PCR products, we recommend using either the QIAquick Gel Extraction Kit (supplied by QIAGEN®) or Centricon® 100 columns (supplied by Amicon®) to remove unincorporated dNTPs and primers. In our experience, other DNA purification kits and methods are less reliable for preparing automated sequencing quality DNA samples. Please call us if you have any questions regarding the compatibility of other DNA sample preparation methods with our DNA sequencers.

Contact Details: 1490 Drew Avenue, Suite 170 Davis California 95616 United States of America Tel: (530) 297-5038 Fax: (530) 297-5039 Email: info@davissequencing.com WWW: www.davissequencing.com/

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