Agilent Offers First FFPE Kit for Oligo CGH Microarrays

Novel Kreatech Labeling Method Enables Microarray Analysis of DNA from Preserved Tumor Samples Considered Too-Damaged Until Now

SANTA CLARA, Calif., and AMSTERDAM, The Netherlands, Aug. 15, 2007 -- Agilent Technologies Inc. (NYSE: A) and Kreatech Biotechnology BV today announced the availability of labeling kits that, for the first time, enable oligo microarray comparative genomic hybridization (CGH) analysis of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples.

The method is based on Kreatech’s Universal Linkage System (ULS) technology, a non-enzymatic direct labeling methodology that has been optimized for Agilent oligo CGH microarrays. Enzymatic labeling can introduce bias while further reducing DNA fragment size. This new labeling technology also features a simple, single-tube protocol, enabling a reduced cost per experiment.

“One of the key advantages of our ULS labeling technology is that it eliminates the bias often found with enzymatic labeling methods, so one can label DNA of any fragment size,” said Dimitri Pappaioannou, senior product manager at Kreatech. “This makes it especially suited for FFPE DNA samples. We have been working closely with Agilent on the development, optimization and validation of the ULS technology on the Agilent oligo aCGH platform, and we are very excited about the results. Through Agilent we are now able to provide researchers with a solution for FFPE samples that were inaccessible in the past.”

“Now researchers can harness the high resolution, genome-wide copy number change profiling capabilities of the Agilent aCGH platform using FFPE samples,” stated Dione Bailey, Agilent CGH product manager. “Given the rapid adoption of CGH versus classical techniques such as spectral karyotyping, FISH, and BAC arrays, we anticipate the release of this kit will further expand this growing market.”

This method addresses an unmet need of clinical researchers. There are an estimated 400 million FFPE-preserved samples in tissue banks worldwide, and the DNA in these samples has been considered too degraded to use in microarray analysis techniques such as aCGH, a powerful method for studying DNA copy number variation in cancers and genetic disorders. As an example, according to the National Cancer Institute’s Web site, the Department of Pathology and Laboratory Medicine at the University of Pennsylvania has approximately 600,000 paraffin-embedded tissue samples that date back to the 1940s. As a result, this labeling capability would allow more comprehensive retrospective analysis from sample biobanks and clinical repositories at a large number of cancer centers.

“Our comparison data showed that the new Agilent non-enzymatic labeling kit is superior to standard Klenow-based DNA labeling protocol,” said Lynda Chin, M.D., Dana-Farber Cancer Institute. “The improved signal achieved with this non-enzymatic labeling technique was more apparent when working with modestly degraded genomic DNA extracted from formalin-fixed paraffin-embedded samples.”

“The Agilent DNA extraction protocol combined with non-enzymatic labeling gave good results from our FFPE samples,” said Chris Jones, Ph.D. in the Pediatric Molecular Pathology Department of the Institute of Cancer Research in Sutton, U.K. “We have a large number of FFPE samples across many tumor types that have been previously inaccessible. As an initial priority we have a series of approximately 90 clinical samples from which data can now be retrieved using this new extraction and labeling approach.”

For more information about the new reagents for use with Agilent oligo CGH microarrays, or the entire Agilent portfolio of microarrays, reagents, instruments and software, please visit www.opengenomics.com.

About Kreatech

Kreatech Biotechnology B.V., a private company founded in 1990, focuses on the creation, development, production and marketing of innovative diagnostic and life science products. Kreatech is committed to achieve and maintain the highest standards in production and quality control as well as in the relationships with our customers and partners. The company’s quality policies are demonstrated by the implementation of ISO 9001 (2000) and ISO 13485 (GMP 2003) guidelines. Information about Kreatech is available on the Web at www.kreatech.com .

About Agilent Technologies

Agilent Technologies Inc. (NYSE: A) is the world’s premier measurement company and a technology leader in communications, electronics, life sciences and chemical analysis. The company’s 19,000 employees serve customers in more than 110 countries. Agilent had net revenue of $5.0 billion in fiscal year 2006. Information about Agilent is available on the Web at www.agilent.com .

# # #

Further technology, corporate citizenship and executive news is available on the Agilent news site at www.agilent.com/go/news .

Press release URL: http://www.agilent.com/about/newsroom/presrel/2007/15aug-ca07048.html

Additional metadata keywords (for Web use only): cancer research, copy number variations, aCGH

---END---