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ETHYLENE
GLYCOL TEST KIT
SIMPLE, FAST,
ACCURATE AND FULLY AUTOMATED
NO INTERFERENCE
FROM
GLYCEROL,
TRIGLYCERIDES,
HEMOGLOBIN, OR BILIRUBIN
DIFFERENTIATES ETHYLENE FROM PROPYLENE GLYCOL
Precision
|
Ethylene Glycol |
Within-Run |
Total Precision |
|
Precision |
|
Mean |
SD |
CV |
SD |
CV |
|
mmol/L |
mmol/L |
% |
mmol/L |
% |
|
1 |
0 |
0 |
0 |
0 |
|
14 |
0 |
0 |
0.538 |
3.916 |
|
25 |
0.471 |
1.866 |
0.625 |
2.49 |
|
36 |
0.422 |
1.247 |
0.79 |
2.275 |
|
41 |
0.422 |
1.022 |
1.546 |
3.506 |
|
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ETHYLENE GLYCOL POISONING
Catachem introduces the VetSpec™ Ethylene Glycol In-Vitro Diagnostic Chemistry reagent test kit. This is the only automated Ethylene Glycol procedure currently available helping
veterinarians quickly identify toxicity and determine if immediate treatment is needed. These reagents have been uniquely formulated
to provide fast and accurate
quantitative
results without the interference from glycerol, triglycerides, hemoglobin, or bilirubin.
An important toxicological problem in clinical diagnosis is
Ethylene Glycol poisoning. When ingested in the form of antifreeze
or other automotive products, Ethylene Glycol results in central
nervous system depression, cardiopulmonary compromise, and renal
insufficiency. Laboratory features of Ethylene Glycol
poisoning include increase anion gap, and increased osmolal gap, calcium oxalate crystaluria, and detectable Ethylene Glycol in
serum.
Catachem Ethylene Glycol procedure is based on the
affinity of the enzyme Glycerol Dehydrogenase (EC 1.1.1.6.) from
bacteria to catalyze the oxidation-reduction reaction of Ethylene
Glycol in the presence of NAD. This two point kinetic procedure
is read at 340nm and the increase in absorbance is directly
proportional to the concentration of Ethylene Glycol in the serum
sample.
Interfering Substances
The following substances have no significant effect
on the accuracy of this Ethylene Glycol procedure at the
concentrations stated.
| Glycerol |
≤ 47 mg/dl |
| Hemoglobin |
≤ 200
mg/dl |
| Triglycerides |
≤ 1000 mg/dl |
| Bilirubin |
≤ 2.2 mg/dl |
Other substances and certain drugs are also known
to influence the Ethylene Glycol values (1-2).
Sensitivity:
Using a pathlength of 1 cm, a D-absorbance of 0.01-0.015 per mmol/L
should be obtained.
Linearity:
This procedure is linear over the range of 0-50 mmol/L.
Precision:
Precision data was obtained using five levels of protein based
controls and following the NCCLS EP5-T2 procedure.
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