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BILE ACIDS LIQUID
REAGENTS, CALIBRATOR CONTROLS
REDUCED HEMOGLOBIN, LIPEMIA INTERFERENCES
LINEARITY TO 250 mmol/L
EXCELLENT WORKING STABILITY
COST-EFFECTIVE
Precision
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BILE ACIDS |
Within-Run |
Total |
|
Precision |
Precision |
|
Mean |
SD |
CV |
SD |
CV |
|
mmol/L |
mmol/L |
% |
mmol/L |
% |
|
25 |
0.77 |
3 |
1.23 |
5.01 |
|
150 |
2.84 |
1.86 |
7.3 |
5.08 |
|
250 |
2.21 |
0.88 |
11.67 |
4.56 |
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BILE ACIDS (LIQUID)
Catachem introduces the VetSpec™ Bile Acids (liquid) In-Vitro
Diagnostic Chemistry reagent test kit. Catachem is pleased
to announce the introduction of our new reagent system with
calibrator and controls.
Catachem Bile Acids procedure is based on the enzymatic procedure
described by Mashige,
et
al.
In this Bile Acids procedure, 3a-hydroxy Bile Acids are converted
to corresponding 3-keto hydroxy Bile Acids by the action of the
enzyme 3a-hydroxysteroid dehydrogenase (3µ-HSDH) with concomitant
reduction of NAD+ to NADH. The NADH thus produced is subsequently
oxidized to NAD+ in a diaphorase-catalyzed reaction where
nitrotetrazolium blue (NBT) is reduced to form a formazan dye,
which has an absorption maximum at 540nm. The intensity of the
color produced is directly proportional to the concentration of
Bile Acids in the serum sample.
Working Reagent Preparation
The Bile Acids Enzyme
Color Reagent and the Bile Acids Activator Reagent are
packaged in ready-to-use form. No preparation is required. Label
these reagents “Working Reagent R1” and “Working Reagent R2”
respectively. Store the Working Reagents at 2-8°C.
When prepared and stored as directed the Working Reagents are
stable for 60 days at 2-8°C.
Interfering Substances
Samples with the following
concentration substances have no significant effect on the
accuracy of this Bile Acids procedure:
Lipemia (Triglycerides)
Ł
1000 mg/Dl
Extremely icteric serum may
produce erroneous results. If this is the case, make a suitable sample dilution with physiological saline and assayed
sample. Multiply result obtained by the dilution factor.
Method Performance
Characteristics
Sensitivity: Using a
pathlength of 1 cm, a Δ-absorbance of 0.0016-0.0024 per mg/ml
should be obtained.
Linearity:
In this procedure there is not significant nonlinearity over the
range of 0-250
mmol/L.
Precision: Precision
data obtained using three levels of protein based controls and
following the NCCLS EP5-T2 procedure (9). The following results
were observed.
Accuracy
Correlation studies were
carried out between this automated Bile Acids method (Y) and a
reference automated Bile Acids procedure based on the 3µ-HSDH
and Diaphorase reactions (X). Canine serum samples were assayed
and the results compared by the least square regression. The
following statistics were observed:
N
= 12
Range = 4.1-171
Mean
Y = 41.88
Mean
X = 43.84
Y = 0.959x – 0.16
r = 0.978
Sy.x = 11.29
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