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NONESTERIFIED FATTY ACIDS REAGENTS, CALIBRATOR &
CONTROLS
2-REAGENT SYSTEM
FULLY AUTOMATED
PRECISION
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NEFA |
Precision |
|
Mean |
SD |
CV |
|
mmol/L |
mmol/L |
% |
|
0.169 |
0.006 |
3.356 |
|
0.443 |
0.0087 |
1.953 |
|
1.02 |
0.0149 |
1.461 |
|
1.516 |
0.0163 |
1.078 |
|
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NONESTERIFIED FATTY ACIDS (NEFA)
Catachem introduces the VetSpec™
Nonesterified Fatty Acids (NEFA) In-Vitro Diagnostic Chemistry
reagent test kit. Catachem is pleased to announce the introduction
of our new reagent system with calibrator and controls.
Most previously used procedures for Nonesterified Fatty Acids were
based on organic solvent extractions, titration and gas liquid
chromatography. These procedures are complicated, time consuming
and restricted to manual assays. Catachem’s NEFA procedure is
based on the enzymatic synthesis of thiols esters of CoA, known as
Acyl-CoA by the activity of Acyl-CoA Synthatase (ACS) in the
presence of ATP and CoA. The Acyl-CoA thus formed is then oxidized
in a second reaction by Acyl-CoA oxidase (ACOX) to produce
2,3-trans-trans-enoyl-CoA and hydrogen peroxide. The hydrogen
peroxide is then quantitated by the oxidative condensation of
N-Ethyl-(3-sulfopropyl) aniline (ALPS) with 4-aminoantipyrine to
produced a quinoneindamine dye with maximum absorption at 550nm.
The increase in absorbance is directly proportional to the
concentration of NEFA in the original serum sample.
Interfering Substances
The following substances have no significant effect on the
accuracy of this NEFA procedure at the concentrations stated.
Hemoglobin ≤ 200 mg/dl
Bilirubin
≤10.0 mg
Ascorbic Acid ≤ 20 mg/dl
Other substances and certain drugs are also known to influence the
NEFA values.
Method Performance Characteristics
Sensitivity: Using a pathlength of 1 cm, a Δ-absorbance of
0.1-0.20 per mmol/L should be obtained.
Linearity: This procedure is linear over the range of 0-2.5
mmol/L.
Precision: Precision data was obtained using five levels of
protein based controls and following the NCCLS EP5-T2 procedure.
The following results were observed:
ACCURACY
Using an automated analyzer, correlation studies were carried out
between this Catachem NEFA procedure (Y) and a commercially
available NEFA test kit as reference (X). Serum samples were
assayed and the results compared by the least squares regression.
The following statistics were observed:
N = 44
Range = 0.1-2.3 mmol/L
Mean Y = 0.5295 mmol/L
Mean X = 0.5841 mmol/L
Y = 0.9137x – 0.0042
r = 0.9949
Sy.x = 0.0541
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