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SORBITOL DEHYDROGENASE (SDH)
Catachem introduces the VetSpec™ Sorbitol
Dehydrogenase (SDH) In-Vitro Diagnostic Chemistry reagent test
kit. Catachem is pleased to announce the introduction of our new
stable liquid reagent system with calibrator and controls.
Measurement of SDH activity is of considerable
clinical value as an effective indicator of acute hepatic
anoxia. Studies by Gertch and others have supported this premise
by reporting sharp increases in serum SDH activity in cases of
extensive liver damage, such as in acute hepatitis.
Several procedures have been reported in the literature for
measuring the enzyme, L-Iditol Dehydrogenase or Sorbitol
Dehydrogenase (SDH, EC 1.1.1.14) in serum. Catachem’s procedure is
based on the enzymatic procedure described by Clive I. Rose and
Arthur R. Henderson. In this procedure SDH catalyzes the
reversible oxidation-reduction reaction between Sorbitol and
Fructose with concomitant oxidation of NADH to NAD+. The decrease
in absorbance is monitored at 340nm. The delta absorbance
produced is directly proportional to the concentration of SDH
activity in the serum sample.
To improve working stability
and preparation of working reagents, Catachem now packages both
SDH reagents as ready to use liquid reagents. These new
reagents have shelf stability to expiration date of the specific
lot number and a working stability of minimum 60 days upon opening
of the SDH Sample Diluent (R1) and Activator Reagent (R2)
containers. To standardize results based on calibration rather
than a theoretical K-Factor, Catachem also offers an SDH
Calibrator along with SDH Controls Level I and Level II. Catachem
recommends the use of our SDH Calibrator on a daily basis to
maintain accuracy and precision of results. Catachem SDH
Calibrator and Controls Level I and Level II now have a minimum of
30 days working stability.
Stability Of New Reagents,
Calibrator and Controls:
Shelf Stability:
To expiration when stored at 2-8ºC.
Working Stability:
Minimum 60 days upon opening of reagent
containers and minimum 30 days upon opening of calibrator and
controls containers and both sets stored at 2-8ºC.
Sensitivity:
Using a pathlength of 1 cm, a
D-absorbance
of 0.0016-0.0024 per mg/ml should be obtained.
Linearity:
In this procedure there is not significant nonlinearity over the
range of 0-100 U/L.
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