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URINE CSF PROTEIN (MICROPROTEIN)
Catachem introduces the VetSpec™ Urine CSF Protein (Microprotein)
In-Vitro Diagnostic Chemistry reagent test kit. Catachem is
pleased to announce the introduction of our new reagent system
with calibrator and controls.
Several methods have
been described for the quantitative determination of Total
Protein in urine and cerebrospinal fluid (CSF). Procedures
utilizing dye-binding suffer from interferences making the
protein assay inaccurate. Other procedures are time consuming,
tedious and unsuitable for automated analysis. Catachem Protein
procedure for urine and CSF is based on the work of Wata I.,
Nishikaze O., subsequently modified by Richard W. Luxton et al. In
this procedure benzathonium chloride reacts with protein to
produce a homogeneous and stable turbidity. The procedure shows
the same reactivity either with albumin or
g-globulin.
Interference from short peptides is not observed and
interference from magnesium is prevented by the presence of EDTA
in the Working Reagent.
Interfering Substances
The following substances
have no significant effect on the accuracy of this Urine CSF
Protein procedure:
| Ascorbic acid |
Cefazolin
Sodium |
Magnesium |
| Calcium |
Glucose |
Phosphorus |
| Creatinine |
Gentamicin Sulfate |
Sodium Citrate |
| Chlorpromazine |
L-Dopa |
Uric Acid |
Method Performance
Characteristics
Sensitivity: Using a
pathlength of 1 cm, a Δ-absorbance
of 0.0016-0.0024 per mg/dl should be obtained.
Linearity: In this
procedure there is no significant nonlinearity over the range
of 0-200 mg/dL (0-2000 mg/L)
Precision: Precision
data was obtained using three levels of protein based controls and
following the NCCLS EP5-T2 procedure. The following results were
observed.
Accuracy
Correlation studies were
carried out between this automated Urine CSF Protein method (Y)
and a reference automated Urine CSF Protein procedure based on the
benzathonium chloride reactions (X). Samples were assayed and the
results compared by the least square regression. The
following statistics were observed:
N = 26
Range = 12-200 mg/dL
Mean Y = 81.35
Mean X = 78.24
Y = 0.995X – 1.201
r = 1.000
Sy.x = 2.02
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