GE Healthcare Launches HiTrap Albumin & IgG Depletion and Albumin & IgG Depletion SpinTrap Prepacked Column

Depletion of albumin and IgG from human serum and plasma allows detection of less abundant proteins

May 18th 2009: GE Healthcare announces the launch of HiTrap™ Albumin & IgG Depletion and Albumin & IgG Depletion SpinTrap™ prepacked columns, for the depletion of albumin and IgG from human serum and plasma. Depleting samples of albumin and IgG allows proteins normally obscured by albumin and IgG to be visualized, greatly enhancing the quality and depth of analysis.

Both columns have high depletion capacity, removing >95% albumin and >90% IgG. Very high reproducibility is achieved, which is an important consideration in parallel sample preparation. Separations are simple and fast, taking approximately 35 minutes to complete with the HiTrap column, for larger sample volumes (~150 µl), and approximately 10 minutes with the SpinTrap column, for smaller sample volumes (~50 µl). In addition, the HiTrap column may be used for a limited number of repeat runs, with consistent depletion rates.

The columns are prepacked with a mixture of anti-HSA Sepharose High Performance and Protein G Sepharose High Performance, in a medium consisting of highly cross-linked agarose beads with covalently immobilized affinity ligands. Samples are processed through the HiTrap column using a liquid chromatography system (e.g. ÄKTA), peristaltic pump, or manually using a syringe, and through the SpinTrap column using a tabletop centrifuge.


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