MADISON, WISCONSIN, USA (October 22, 2007) Promega
Corporation, a leading provider to the life sciences industry, announced the
launch of its HaloCHIP™ System the first antibody-free alternative to
chromatin immunoprecipitation (ChIP).
ChIP is a method used to determine where, in vivo, a given protein binds to
DNA. The major challenge of the ChIP method is the requirement for highly
specific antibodies for each protein to be tested. The HaloCHIP System is
the first to work without the use of antibodies.
In addition to being antibody-free, HaloCHIP delivers results in less time
than standard ChIP (2 days vs. 4 days). The HaloCHIP system also improves
signal-to-noise ratios, enabling higher sensitivity for detection of even
small changes in protein binding patterns from a reduced number of cells.
The HaloCHIP System is based on HaloTag® Technology from Promega. The
HaloTag reporter protein has been engineered to form a covalent interaction
with a series of specific ligands, including one for immobilization,
HaloLink Resin. In HaloCHIP, DNA-binding proteins of interest are expressed
in cells as HaloTag fusion proteins, crosslinked to DNA with formaldehyde,
and then covalently captured on HaloLink Resin. After the captured complexes
are rigorously washed to remove any non-specific interactions, crosslinks
are reversed, and the specific DNA is released for downstream analysis.
HaloCHIP is the most recent addition to the Promega line of integrated tools
for functional proteomics, which currently center on three proprietary
technologies:
- HaloTag Multifunctional Technology – based on
the use of a single gene fusion construct for protein expression and
purification, imaging, and immobilization applications.
- TNT® Expression Systems – convenient,
single-tube, coupled transcription/translation reactions for eukaryotic
cell-free protein expression.
- Flexi® Vector Systems – simple, yet powerful,
directional cloning method for protein-coding sequences.
Commenting on the launch of HaloCHIP, Danette
Hartzell, Promega R&D Scientist said: “The HaloCHIP System means that
scientists no longer need to struggle to find the perfect antibody for their
ChIP experiments. Since HaloCHIP is based on HaloTag Technology, researchers
can easily run additional protein analysis applications, including cellular
imaging and protein:protein interactions, using only one genetic construct.
That yields both consistency and time savings.”
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Contacts
Promega
Penny Patterson
Director, Corporate Affairs
Tel: +1 608 274 4330
E-mail: penny.patterson@promega.com
Northbank Communications
Lorna Cuddon, Katie Odgaard, Claire Mosley
Tel: +44 (0)20 7268 3002
E-mail: k.odgaard@northbankcommunications.com
About Promega
Promega Corporation is a leader in providing innovative solutions and
technical support to the life sciences industry. The company’s 1,450
products enable scientists worldwide to advance their knowledge in human
identification, genomics, proteomics, cellular analysis, and molecular
diagnostics. Visit www.promega.com
for more information.
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