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One-step Cell Lysis and Protein Purification
ST. LOUIS, MO,
and Taufkirchen, Germany, 12th January 2006 - Sigma, a
division of Sigma-Aldrich Corporation (Nasdaq: SIAL), has
launched a new HIS-Select™ iLAP™ 5 ml Column (Product code H9913). The
HIS-Select iLAP column is an innovative, patent-pending method that combines
cell lysis and histidine- tagged protein purification steps into one step,
allowing fast, efficient and non-denaturing protein extraction directly from
microbial cultures.
The HIS-Select iLAP column consists of a novel
single-use, disposable column designed for the one-step purification of
histidine-tagged proteins directly from a 5 ml bacterial culture. This product
maximizes the speed and efficiency of recombinant protein extraction while
eliminating sample loss. The column contains highly specific HIS-Select™
nickel chelate matrix and highly efficient CelLytic™ Express, both present in
a convenient tablet format. The column is designed for the simultaneous,
direct lysis of a 5 ml bacterial culture and affinity capture of the target
histidine-tagged protein without any additional manipulation required (such as
centrifugation). As the culture is lysed in the column, the target protein is
captured on the same highly selective HIS-Select Affinity Gel. The relatively
high capacity feature of these columns enables capture of at least 1-2 mg of
histidine-tagged protein per column, making them ideal for confirming
expression of a histidine-tagged target protein, for performing
protein:protein interaction assays, or for rapidly screening colonies or
multiple constructs (cultures). The resulting purified protein is compatible
with protein assays, SDS-PAGE, Western blotting and MALDI.
"The HIS-Select iLAP technology has dramatically
simplified purification of histidine-tagged recombinant proteins directly from
bacterial cultures," said Michael Hadjisavas, Global Strategic Marketing
Manager, Proteomics, at Sigma-Aldrich Corporation. "This new 5 ml column
format allows for the rapid screening of recombinant clones for protein
expression and subsequent protein characterization," he added. "Since this
technology allows for simultaneous cell lysis and protein purification, it
will significantly reduce purification time as well as sample handling,
minimizing the potential for protein loss or damage. As a leader in protein
purification, we have taken efficiency one step further by introducing a
technology which improves the speed and efficiency by which recombinant
proteins can be purified."
About
Sigma-Aldrich
Sigma-Aldrich is a leading Life
Science and High Technology company. Our biochemical and organic
chemical products and kits are used in scientific and genomic research,
biotechnology, pharmaceutical development, the diagnosis of disease and as key
components in pharmaceutical and other high technology manufacturing. We have
customers in life science companies, university and government institutions,
hospitals, and in industry. Over one million scientists and technologists use
their products. Sigma-Aldrich operates in 35 countries and has 6,800 employees
providing excellent service worldwide. We are committed to accelerating
Customer's success through leadership in Life Science, High
Technology and Service. For more information about Sigma-
Aldrich, please visit its award-winning web site at
http://sigma-aldrich.com.
Media enquiries in Europe to Northbank Communications: Marc Egelhofer, Tel: +44 (0)1260 296505 m.egelhofer@northbankcommunications.com At Sigma-Aldrich:
Michael Hadjisavas,
+1-314-286-7431
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