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BioStatus - Simple flow-based total nucleated cell differential
A new method
published in Cytometry part B uses DRAQ5 to gate all nucleated cells, FlowCount
beads, a panel of 6 antibodies in 4 clusters and the 5 fluorescence channels
plus forward and side scatter on the Beckman Coulter FC500 flow cytometer to get
a comprehensive analysis of both nucleated cells and platelets in blood or bone
marrow samples. Ten separate classes of nucleated cells could be identified.
The one-tube, lyse-no wash method gave excellent correlation to both cell
counters and microscopy and offers a real alternative to the laborious manual
scoring of blood smears from samples flagged by the cell counter. Using the DNA
binding properties of DRAQ5 cell cycle information was also available whilst
gating on the DRAQ5-/CD36+ events permitted enumeration of platelets. This
method could offer an effective replacement for the subjective, labour-intensive
and statistically-limited analysis by manual microscopy.
Biostatus Ltd.
+44 (0)1509 558 163
enquiry@biostatus.com
www.biostatus.com
http://www.biostatus.com/resources/articles/total_nucleated_cell_differential/
Press contact
Roy
Edward
Sales & Mktg. Director, Biostatus Ltd
SKYPE: roy.edward.biostatus MOB: 07765 533615 TEL: 01509 558163;
roy@biostatus.com; www.biostatus.com
Co. #: 3079239. Regd. in England & Wales. Regd. office: 56 Charnwood Road,
Shepshed, LE12 9NP, UK.
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