Methods in Molecular Biology Course

Course Description:

This course is an introduction to molecular biology, with a focus on the techniques used to isolate, identify, modify and analyze three key molecules: DNA, RNA and proteins. The course describes the principle concepts and uses of each technique, using a case study to illustrate the relevant points. The course does not cover the protocols for these techniques.

Course Objectives:

> Describe the basic structure and function of DNA, RNA and Proteins

> Explain the "Central Dogma"

> Understand the steps in the translation of RNA to protein

> Explain how DNA is manipulated

> Describe how a fragment of DNA is cloned

> List three methods of introducing DNA into cells

> Explain how recombinant proteins are generated

> Explain how DNA is sequenced

> List the reagents needed to perform PCR

> Explain the concept of PCR

> Explain how RT-PCR is used for gene expression analyses

> List and explain the four common types of "real time PCR"

> Explain how RNA and DNA are separated using electrophoresis

> Explain how proteins are separated using electrophoresis

> Explain how large mixtures of proteins can be analyzed

> Specify the main steps of chromatography

> List and describe the five types of chromatography

> Explain how Southern, northern and western blotting are performed

> Specify the uses of each technique

Course Outline:

Introduction

1. Course information

2. Course objectives

3. Course overview

Fundamentals

4. Objectives

5. Central Dogma

6. DNA/RNA structure

7. Translation

8. Protein structure

9. Progress check 1

Cloning

10. Objectives

11. DNA manipulation

12. DNA cloning and gene transfer

13. DNA sequencing

14. Recombinant proteins

15. Case study 1

16. Progress Check 2

Polymerase Chain Reaction

17. Objectives

18. PCR

19. Real time PCR

20. RT-PCR

21. Case study 2

22. Progress Check 3

Separation Techniques

23. Objectives

24. DNA and RNA Electrophoresis

25. SDS-PAGE

26. Native gels

27. IEF

28. 2D Gel Electrophoresis

29. Chromatography

30. Case study 3

31. Progress Check 4

Blotting Techniques

32. Objectives

33. Southern and Northern

34. Western

35. Case study 4

36. Progress Check 5

Conclusions

37. Summary

Each Online Training Course or Professional Designation Program listed below comes complete with;

> 24 hour access to the course for 90 days,

> a printable PDF file including the notes to the course,

> a test used to measure your understanding of the material provided in the course, and

> a Certificate of Completion once you have passed a course, or;

> a Certificate of Professional Designation if you are enrolling in a Professional Designation program

Course Unit 2 of 5 Units
Price: $245.00 / GBP127.40