Microarrays

Course Description:

The microarrays course describes the various chip fabrication techniques, image and data analysis, and the uses of microarrays for re-sequencing, expression studies and genotyping.

Instructors:

Dr. Raji Pillai received her B.A. in Biochemistry from Smith College, and in 1994, her Ph.D. in Biological Chemistry from UCLA. She started work in the microarray field during a post-doctoral fellowship in Dr.Patrick Brown's laboratory at Stanford University. She continues work in this area as a scientist in the biotechnology industry.

Dr. Jay Wohlgemuth received his B.Sc. from Harvard University, and his M.D. from Stanford University. He completed his medical residency at Washington University. He began working on microarrays in Dr. Tom Quertermous's laboratory at Stanford University, and is now a Principal scientist at BioCardia, a company focused on cardiovascular genomics.

Course Objectives:

> Explain the relevance of microarray technology in genomics.

> Describe how different kinds of microarrays are fabricated.

> Describe how microarray experiments are performed.

> Explain how microarrays are scanned, and how the fluorescent output is analyzed to generate quantitative data.

> Explain how bioinformatics is used in microarray data analysis.

> Understand how microarrays are currently used

Course Outline:

Introduction

1. Course Description

2. Historical Perspective

3. Course Overview

Probes

4. Objectives

5. Types of Probes

6. Progress Check

Array Fabrication

7. Objectives

8. Photolithography

9. In Situ Oligonucleotides

10. Oligonucleotide Deposition

11. cDNA Preparation

12. DNA Arrays

13. Array Surfaces

14. Progress Check

Targets

15. Objectives

16. Types and Labeling

17. RNA Amplification

18. Progress Check

Assay

19. Objectives

20. Hybridization Conditions

21. Progress Check

Readout

22. Objectives

23. Why Fluorescence?

24. Scanning Technology

25. Progress Check

Image Analysis

26. Objectives

27. Quantification

28. Normalization

29. Informatics

30. Cluster Analysis

31. Progress Check

Uses/Examples

32. Objectives

33. Sequencing

34. Genotyping-HIV

35. Expression Analyses

36. Liquid Arrays-MPSS

37. Progress Check

Conclusion

39. Summary and Future Trend

40. Final Assessment

Each Online Training Course or Professional Designation Program listed below comes complete with;

> 24 hour access to the course for 90 days,

> a printable PDF file including the notes to the course,

> a test used to measure your understanding of the material provided in the course, and

> a Certificate of Completion once you have passed a course, or;

> a Certificate of Professional Designation if you are enrolling in a Professional Designation program
Course Unit 5 of 5 Units
Price: $245.00 / GBP134.75