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DAVIS SEQUENCING, INC. Company Profile

09:38 EDT 22nd September 2017 | BioPortfolio

We offer DNA sequencing of single stranded or double stranded DNA samples from purified plasmids and PCR products. Our sequencing reactions are performed using the Applied Biosystems Big Dye Terminator V3.0 sequencing chemistry. One sequencing reaction consists of one DNA template (customer supplied plasmid DNA or PCR product) being sequenced with one primer (customer supplied primer or one of our supplied primers). We supply several frequently used sequencing primers for free, but the template DNA samples are supplied by our customers.
Our sequencing reaction read-lengths are normally between 800 and 900 bases (with a basecalling accuracy >99%) when run on our ABI 3730 DNA sequencers. Sequencing read-lengths are dependent upon several factors including template DNA concentration, purity and base composition (poly A and/or T regions, GC content and short tandem repeats) as well as primer binding specificity.

Since DNA sample purity and concentration are the two most critical factors to the success of automated sequencing reactions, we highly recommend that customers use the QIAGEN® plasmid DNA prep kits to purify their plasmid DNA samples. For purifying PCR products, we recommend using either the QIAquick Gel Extraction Kit (supplied by QIAGEN®) or Centricon® 100 columns (supplied by Amicon®) to remove unincorporated dNTPs and primers. In our experience, other DNA purification kits and methods are less reliable for preparing automated sequencing quality DNA samples. Please call us if you have any questions regarding the compatibility of other DNA sample preparation methods with our DNA sequencers.

Location

1490 Drew Avenue, Suite 170
Davis
California
95616
United States of America

Contact

Phone: (530) 297-5038
Fax: (530) 297-5039
Email: info@davissequencing.com


News Articles [611 Associated News Articles listed on BioPortfolio]

University of California Davis, Tempus Partner on Personalized Cancer Initiative

University of California Davis Comprehensive Cancer Center is collaborating with technology company Tempus on a precision medicine partnership. The new collaboration will focus on advancing clinical c...

Tempus, U of California Davis ink personalized medicine partnership

Tempus, a technology company focused on helping doctors personalize cancer care by collecting and analyzing large volumes of molecular and clinical data, and University of California Davis Comprehensi...

Author Raymond Davis’ Newly Released “Jesus Confirmed Raymond Davis 100% Proof” Recounts the Author’s Divine Encounter, which Reaffirmed his Faith

(PRWEB) July 14, 2017 “Jesus Confirmed Raymond Davis 100% Proof”: a confirmation of the Lord Jesus Christ. “Jesus Co...

Europe DNA Sequencing Market Forecast 20172023 [Updated: 04012017] Prices from USD $1

LIST OF FIGURES FIGURE1 EUROPE DNA SEQUENCING MARKET 20172023 $ MILLIONFIGURE2 EUROPE DNA SEQUENCING MARKET BY GEOGRAPHY 2016 $ MILLIONFIGURE3 EUROPE DNA SEQUENCING MARKET SHARE ANALYSIS 2...

Next Gen Sequencing Challenges

Next generation sequencing opened the doors to our genome. It gives massive amounts of information in a week – whereas Sanger sequencing takes thrice as long, and causes lab lesions due to the abusi...

$1.6M from NSF to UC Davis to study water, land use in disadvantaged communities

(University of California - Davis) The National Science Foundation has awarded $1.6M to the University of California, Davis to analyze the complex relationships between surface water and groundwater s...

An Introduction to Nanopore Sequencing

DNA sequencing is the most powerful method to reveal genetic variations at the molecular level, leading to a better understanding of our body in physiological settings, and pathological conditions. It...

Maxam-Gilbert Sequencing: What Was It, and Why It Isn’t Anymore

In the mid-1970s, two methods were developed for directly sequencing DNA: the Maxam-Gilbert sequencing (or chemical sequencing) method and the Sanger chain-termination method. Indeed, in 1980, both Wa...

PubMed Articles [923 Associated PubMed Articles listed on BioPortfolio]

GLM-based optimization of NGS data analysis: A case study of Roche 454, Ion Torrent PGM and Illumina NextSeq sequencing data.

There are various next-generation sequencing techniques, all of them striving to replace Sanger sequencing as the gold standard. However, false positive calls of single nucleotide variants and especia...

Correction for Xu and Davis, "c-Jun NH2-Terminal Kinase Is Required for Lineage-Specific Differentiation but Not Stem Cell Self-Renewal".

Pan-Cancer Analysis of Genomic Sequencing Among the Elderly.

We hypothesized that elderly patients might have age-specific genetic abnormalities yet be underrepresented in currently available sequencing repositories, which could limit the effect of sequencing e...

A primer to clinical genome sequencing.

Genome sequencing is now available as a clinical diagnostic test. There is a significant knowledge and translation gap for nongenetic specialists of the processes necessary to generate and interpret c...

Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area.

PacBio RS II is the first commercialized third-generation DNA sequencer able to sequence a single molecule DNA in real-time without amplification. PacBio RS II's sequencing technology is novel and uni...

Clinical Trials [312 Associated Clinical Trials listed on BioPortfolio]

Enabling Personalized Medicine Through Exome Sequencing in the U.S. Air Force

The MilSeq Project is a nonrandomized, prospective pilot study of whole exome sequencing (WES) in the U.S. Air Force. The purpose of this study is to explore the implementation of WES into...

Bioequivalence Study of Gabapentin 800 mg Tablets Under Fed Conditions

This study compared the relative bioavailability (rate and extent of absorption) of Gabapentin tablets 800 mg by Ranbaxy Laboratories Limited with that of Neurontin ® 800 mg tablets of Pa...

Bioequivalence Study of Gabapentin 800 mg Tablets Under Fasting Conditions

This study compared the relative bioavailability (rate and extent of absorption) of the test Gabapentin tablets 800 mg by Ranbaxy Laboratories Limited to the reference Neurontin ® 800 mg ...

Genomic Sequencing in Patients With HCM Undergoing Septal Myectomy

Investigators aim to use comparative exome and/or genome sequencing to discover causative molecular lesions for phenotypes hypothesized to be caused by somatic mutations. For this study, i...

Genomic Sequencing in Acutely Ill Neonates

The purpose of this study is to compare the effectiveness of rapid next generation sequencing (NGS, such as whole genome sequencing1) with current practice to provide diagnostic or prognos...

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Davis Vision

Headquartered in Plainview, N.Y., Davis Vision is one of America's leading vision benefits companies, providing comprehensive vision benefits for nearly 50 years. More than 55 million individuals nati...

Davis Phinney Foundation

The Davis Phinney Foundation was created in 2004 by cycling legend Davis Phinney. Davis Phinney remains the race leader in victories by an American cyclist, and he is a two-time Tour de France stage w...

DAVIS SEQUENCING, INC.

We offer DNA sequencing of single stranded or double stranded DNA samples from purified plasmids and PCR products. Our sequencing reactions are performed using the Applied Biosystems Big Dye Terminato...

Davis Vision, Inc.

Davis Vision is part of the HVHC Inc. group of companies, which also includes Viva International Group and Eye Care Centers of America. One of the nation's premier managed vision care companies, Davis...

More Information about "DAVIS SEQUENCING, INC." on BioPortfolio

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DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...

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