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ImmunoassayAn immunoassay is a biochemical test that measures the concentration of a substance in a biological liquid, typically serum or urine, using the reaction of an antibody or antibodies to its antigen. The assay takes advantage of the specific binding of an antibody to its antigen. Monoclonal antibodies are often used as they only usually bind to one site of a particular molecule, and therefore provide a more specific and accurate test, which is less easily confused by the presence of other molecules. The antibodies picked must have a high affinity for the antigen .Both the presence of antigen or antibodies can be measured. For instance, when detecting infection the presence of antibody against the pathogen is measured. For measuring hormones such as insulin, the insulin acts as the antigen.For numerical results, the response of the fluid being measured must be compared to standards of a known concentration. This is usually done though the plotting of a standard curve on a graph, the position of the curve at response of the unknown is then examined, and so the quantity of the unknown found. Detecting the quantity of antibody or antigen can be achieved by a variety of methods. One of the most common is to label either the antigen or antibody. The label may consist of an enzyme ), radioisotopes such as I-125 Radioimmunoassay , magnetic labels or fluorescence. Other techniques include agglutination, nephelometry, turbidimetry and Western Blot.Immunoassays have a particularly important role in the diagnosis of HIV through the HIV test (From the Wikpedia article Immunoassay.) Download PDF Image ResultsLoading...
BioPortfolio Ltd. offers e-mail and postal lists for Immunoassay scientists - we have details of around 4200 individuals working on Immunoassay . This page has been viewed 79 times Recent Search Terms used to find this page: . Browse BioPortfolio's InDepth service - alphabetically: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z or by Most Publications, recently searched for, or most viewed. Search for Immunoassay across BioPortfolio, or bestselling Immunoassay books or recently published Immunoassay books . Wikipedia excerpt, where present, licenced under the GNU Free Documentation License. Resources from the NCBI applied. Selected MeSH subject headings created and maintained by the US NLM are used in conjunction with additional keywords. 2006-2008 MeSH. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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