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Immunoassay

An immunoassay is a biochemical test that measures the concentration of a substance in a biological liquid, typically serum or urine, using the reaction of an antibody or antibodies to its antigen. The assay takes advantage of the specific binding of an antibody to its antigen. Monoclonal antibodies are often used as they only usually bind to one site of a particular molecule, and therefore provide a more specific and accurate test, which is less easily confused by the presence of other molecules. The antibodies picked must have a high affinity for the antigen .Both the presence of antigen or antibodies can be measured. For instance, when detecting infection the presence of antibody against the pathogen is measured. For measuring hormones such as insulin, the insulin acts as the antigen.For numerical results, the response of the fluid being measured must be compared to standards of a known concentration. This is usually done though the plotting of a standard curve on a graph, the position of the curve at response of the unknown is then examined, and so the quantity of the unknown found. Detecting the quantity of antibody or antigen can be achieved by a variety of methods. One of the most common is to label either the antigen or antibody. The label may consist of an enzyme ), radioisotopes such as I-125 Radioimmunoassay , magnetic labels or fluorescence. Other techniques include agglutination, nephelometry, turbidimetry and Western Blot.Immunoassays have a particularly important role in the diagnosis of HIV through the HIV test (From the Wikpedia article Immunoassay.)

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Recent Publications on Immunoassay:

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Interaction of Digibind with endogenous cardiotonic steroids from preeclamptic placentae.
BACKGROUND: Preeclampsia is a major cause of maternal and fetal mortality,...
21st November, 2009
aLaboratory of Cardiovascular Science, National Institute on Aging, J Hypertens. 2009 Nov 18.
DOI Direct Link
Validation of a Total Testosterone Assay Using High Turbulence Liquid Chromatography Tandem Mass Spectrometry: Total and Free Testosterone Reference Ranges.
Accurate measurement of testosterone concentration is of critical...
21st November, 2009
Departments of Endocrinology and Metabolism, Quest Diagnostics Nichols Steroids. 2009 Nov 16.
DOI Direct Link
Quantum Dots Enhanced Ultrasensitive Detection of DNA Adducts.
Here we demonstrate that quantum dots (QD) can greatly improve the...
21st November, 2009
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Anal Chem. 2009 Nov 19.
DOI Direct Link
Evaluation of anti-ribosomal P protein immunoassay in Japanese patients with connective tissue diseases: comparison with an indirect immunofluorescence assay.
Objectives: To investigate the prevalence of anti-ribosomal P protein...
20th November, 2009
Division of Connective Tissue Disease and Autoimmunity, Department of Scand J Rheumatol. 2009;38(6):460-463.
Development of high-throughput chemical extraction techniques and quantitative HPLC-MS/MS (SRM) assays for clinically relevant plasma proteins.
The clinical application of targeted plasma protein analysis by selective...
20th November, 2009
J Proteome Res. 2009 Nov 18.
DOI Direct Link

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Press Releases About Immunoassay:

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Reportlinker Adds Cholesterol and Other Cardiovascular Testing - Trends & Opportunities Worldwide 25th November, 2009 Reportlinker
NEW YORK, Nov. 25 /PRNewswire/ -- Reportlinker.com announces that a new market research report is available in its catalogue: Cholesterol and Other Cardiovascular Testing - Trends & Opportunities
Reportlinker Adds Biotechnology Reagents - A Global Market Perspective 18th November, 2009 Reportlinker
NEW YORK, Nov. 18 /PRNewswire/ -- Reportlinker.com announces that a new market research report is available in its catalogue. Biotechnology Reagents - A Global Market Perspective http://www
Calbiotech Awarded U.S. Army Phase I SBIR Research Contract to Develop Analyte Specific Reagents for the Diagnosis of Dengue Fever Virus 6th November, 2009 Calbiotech, Inc.
SPRING VALLEY, Calif., Nov. 6 /PRNewswire/ -- Calbiotech, Inc. announced today that it has been awarded a Small Business Innovative Research (SBIR) Phase I contract by the U.S. Army Medical Research
Reportlinker Adds Point of Care Diagnostics 2010 and Beyond: Rapid Testing at a Crossroads 5th November, 2009 Reportlinker
NEW YORK, Nov. 5 /PRNewswire/ -- Reportlinker.com announces that a new market research report is available in its catalogue. Point of Care Diagnostics 2010 and Beyond: Rapid Testing at a Crossroads
Beckman Coulter Announces Third Quarter 2009 Results 29th October, 2009 Beckman Coulter, Inc.
Three Months Ended Nine Months Ended September 30 September 30 ------------

BioNews Results for Immunoassay:

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Reportlinker Adds Biotechnology Reagents - A Global Market Perspective
Charlotte Business Journal:  Nov 18 2009 6:45PM Matching: immunoassay
Calbiotech Wins Phase I SBIR Contract to Develop Blood-Based Dengue Fever Diagnostic
Genetic Engineering News:  Nov 7 2009 2:44AM Matching: immunoassay
Calbiotech Awarded U.S. Army Phase I SBIR Research Contract to Develop Analyte Specific Reagents for the Diagn
KOTA Territory News:  Nov 6 2009 10:13PM Matching: immunoassay
Calbiotech Awarded U.S. Army Phase I SBIR Research Contract to Develop Analyte Specific Reagents for the Diagn
Montana's News Station:  Nov 6 2009 8:59PM Matching: immunoassay
Calbiotech Awarded U.S. Army Phase I SBIR Research Contract to Develop Analyte Specific Reagents for the Diagn
KSLA:  Nov 6 2009 7:18PM Matching: immunoassay
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Immunoassay Patents:

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US Patent No.Title
7615351 Two stage nucleic acid amplification using an amplification oligomer
7618392 Methods and compositions for detection of microbial contaminants in peritoneal dialysis solution
7618560 Stable metal/conductive polymer composite colloids and methods for making and using the same
7618587 Analyzers and methods of analyzing blood
7618590 Fluid dispensing system

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Companies working in the area of Immunoassay from Bioportfolio's Biocorporate database :

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Company Name

A. F. Schuetzdeller Biochemicals

AFSBIO distributes growth fators and antibodies, with a special emphasis on Insulin-like Growth Factor (IGFs) and IGF Binding Proteins (IGFBPs). Products that have been develloped in house are...
www.afsbio.de/        View full profile
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ABION GmbH

ABION has developed an immunoassay technology ABICAP (Antibody Immuno Column for Analytical Processes), a new generation of immunoassays that is based on an immuno affinity column design. The...
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ADGEN Diagnostic Systems

ADGEN has three main areas of business. First plant disease diagnostics; second, the detection of mycotoxins and microbiological contaminants in foods and feedstuffs and third, the development of...
www.adgen.co.uk/        View full profile
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BioPortfolio Ltd. offers e-mail and postal lists for Immunoassay scientists - we have details of around 4200 individuals working on Immunoassay .


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Wikipedia excerpt, where present, licenced under the GNU Free Documentation License. Resources from the NCBI applied. Selected MeSH subject headings created and maintained by the US NLM are used in conjunction with additional keywords. 2006-2008 MeSH. Thumbshots from Thumbshots.org

 

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