Immunoelectrophoresis

Immunoelectrophoresis is the electrophoresis of a determined antigen mixture in an agarose gel that allows the separation of different proteins along the gel slide, and then the lateral diffusion in the gel of an immune serum or a monoclonal antibody. Antibodies specific to the antigens form prepicitation arcs seen over a dark background, or by Coomassie blue staining.This method was very useful to determine the number of antigens recognised against a particular mosaic antigen but today the Western blot method is preferred because the apparent molecular weight of each antigen can be determined and it is more sensitive, but IEF are still useful when non reducing conditions are needed. (From the Wikpedia article Immunoelectrophoresis.)

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Recent Publications on Immunoelectrophoresis:
Assays for complement proteins encoded in the class III region of human MHC. Investigation of the complement proteins encoded in the class III region...	27th June, 2008	Semmelweis Medical University, Budapest, Hungary.	Curr Protoc Immunol. 2005 Jul;Chapter 13:Unit 13.7. DOI Direct Link
Diagnostic values of beta-2 transferrin and beta-trace protein as markers for cerebrospinal fluid fistula. INTRODUCTION: During recent decades, beta2-transferrin and beta-trace...	26th June, 2008	Rikshospitalet Medical Centre, Oslo, Norway.	Rhinology. 2008 Jun;46(2):82-5.
[Transferrin microheterogeneity in patients treated by maintenance haemodialysis] Transferrin (Tf) plays a crucial role in iron transport in the human body....	25th June, 2008	Katedra i Klinika Nefrologii, Transplantologii i Chorob Wewnetrznych,	Przegl Lek. 2007;64(7-8):483-8.
Chronic inflammation and accelerated atherosclerosis as important cofactors in nephrogenic systemic fibrosis following intravenous gadolinium exposure. Nephrogenic systemic fibrosis (NSF) is a rare disorder in patients with...	14th June, 2008	Clinic of Internal Medicine, Section of Nephrology, HELIOS-Klinikum	Clin Exp Nephrol. 2008 Jun 14;. DOI Direct Link
Urine protein electrophoresis and immunoelectrophoresis using unconcentrated or minimally concentrated urine samples. Our objective was to evaluate a gel system that uses unconcentrated urine...	14th June, 2008	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester,	Am J Clin Pathol. 2008 Jul;130(1):141-5. DOI Direct Link
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Immunoelectrophoresis Clinical Trials:
A Phase II Study of Perifosine in Patients With Relapsed/Refractory Waldenström's Macroglobulinemia	Waldenström's Macroglobulinemia

Immunoelectrophoresis Patents:
US Patent No.	Title
7335488	Fluorescent proteins
7358231	Pancreatic cancer secreted targets and uses thereof
7361481	Diagnosis of lysosomal storage disorders using saposins and other markers
7361736	Family 44 xyloglucanases
7364732	Method for treating inflammation
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