Immunoprecipitation

Immunoprecipitation is the technique of precipitating an antigen out of solution using an antibody specific to that antigen. This process can be used to enrich a given protein to some degree of purity. Co-immunoprecipitation can identify interacting proteins or protein complexes present in cell extracts: by precipitating one protein believed to be in a complex, additional members of the complex are captured as well and can be identified. The protein complexes, once bound to the specific antibody, are removed from the bulk solution by capture with an antibody-binding protein attached to a solid support such as an agarose bead. These antibody-binding proteins were initially isolated from bacteria and recognize a wide variety of antibodies. Following the initial capture of a protein or protein complex, the solid support is washed several times to remove any proteins not specifically and tightly bound through the antibody. After washing, the precipitated protein are eluted and analyzed using gel electrophoresis, mass spectrometry, western blotting, or any number of other methods for identifying constituents in the complex. Thus, co-immunoprecipitation is a standard method to assess protein-protein interaction. Immunoprecipitation is very useful in chromatin immunoprecipitation, which allows analysis of DNA sequences bound to histones or other chromatin components. (From the Wikpedia article Immunoprecipitation.)

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Recent Publications on Immunoprecipitation:
Implications of tyrosine phosphoproteomics in cervical carcinogenesis. ABSTRACT: BACKGROUND: Worldwide cervical cancer remains a leading cause of...	19th July, 2008		J Carcinog. 2008 Jul 17;7(1):2. DOI Direct Link
Regulation of microtubule nucleation from membranes by complexes of membrane-bound gamma-tubulin with Fyn kinase and phosphoinositide 3-kinase. Molecular mechanisms controlling microtubule formation in cells with...	19th July, 2008		Biochem J. 2008 Jul 17;. DOI Direct Link
Persistence of Collagen Type II Synthesis and Secretion in Rapidly Proliferating Human Articular Chondrocytes In Vitro. Articular chondrocytes (AC) expanded in vitro for tissue engineering...	19th July, 2008	Institute of Immunology, Rikshospitalet University Hospital, Oslo, Norway.	Tissue Eng Part A. 2008 Jul 17;. DOI Direct Link
De-repression of the p21 promoter in prostate cancer cells by an isothiocyanate via inhibition of HDACs and c-Myc. Natural isothiocyanates from cruciferous vegetables have been described as...	19th July, 2008	NYU Cancer Institute, New York University School of Medicine, New York, NY	Int J Oncol. 2008 Aug;33(2):375-80.
Changes in expression of imprinted genes following treatment of human cancer cell lines with non-mutagenic or mutagenic carcinogens. It remains possible that chemicals that act by mutagenic mechanisms as...	19th July, 2008	Oral and Maxillofacial Surgery, The Nippon Dental University Hospital at	Int J Oncol. 2008 Aug;33(2):351-60.
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Immunoprecipitation Clinical Trials:
Study of the Role of Tissular Maternofetal Alloimmunization in Placentation Pathologies	Recurrent Pregnancy Losses;   Preeclampsia;   Intervillositis

Immunoprecipitation Patents:
US Patent No.	Title
7393661	Nucleic acids encoding OBG3 polypeptides
7393936	Method for the recombinant production and purification of protein kinases
7393943	Polynucleotides encoding a human chemotactic cytokine I
7393944	T-bet compositions and methods of use thereof
7393950	Antisense oligonucleotides targeted to human CDC45
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Companies working in the area of Immunoprecipitation from Bioportfolio's Biocorporate database :
Company Name
Lab Vision Corporation Information on antibody applications, such as immunohistochemical (IHC) staining and tumor classification. Explore complete protocols and troubleshooting tips for Western blots or immunoprecipitations... www.isinet.com/        View full profile
Bethyl, Inc Bethyl Laboratories, Inc. Since 1972, Bethyl has provided quality antibody products and services for both clinical and research applications. We remain committed to manufacturing new and novel... www.bethyl.com/        View full profile
BioLegend BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value. Our product areas include cell immunophenotyping,... www.biolegend.com/        View full profile
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