BioPortfolio Biotechnology Pharmaceutical Healthcare Medical Life Science Drug Discovery Disease
Search BioPortfolio:       

Radioimmunoassay

Radioimmunoassay is a scientific method used to test antigens without the need to use a bioassay. It involves mixing known quantities of radioactive antigen with antibody to that antigen, then adding unlabeled or "cold" antigen and measuring the amount of labeled antigen displaced.Initially, the radioactive antigen is bound to the antibodies. When "cold" antigen is added, the two compete for antibody binding sites - at higher concentrations of "cold" antigen, more of it binds to the antibody, displacing the radioactive variant. The bound antigens are separated from the unbound ones.One method of separation used initially was the use of a second antibody directed against the first for precipitation and centrifugation. The use of charcoal suspension for precipitation was extended but replaced later by Drs. Werner and Acebedo at Columbia University for RIA of T3 and T4. An ultramicro RIA for human TSH was published in BBRC by Drs. Acebedo, Hayek et al. The free hormone stays in the supernatant, the radioactivity of which is measured and a binding curve is plotted. The technique is both extremely sensitive and specific, but it requires special precautions , sophisticated apparatus, and is expensive. The technique has been largely replaced by the ELISA method where instead of a radioactive signal, the antigen-antibody reaction is measured by colorometric signals. The RIA was developed by Rosalyn Yalow and Solomon Aaron Berson in the 1950s. Rosalyn Sussman Yalow received the 1977 Nobel Prize in Medicine for the development of the RIA for insulin. The precise measurement of minute amounts of such a hormone was a key event in endocrinology. (From the Wikpedia article Radioimmunoassay.)

Download PDF PDF Version

Image Results

Loading...

Recent Publications on Radioimmunoassay:

Pubmed Logo
GAD65 IgG autoantibodies in stiff person syndrome: clonality, avidity and persistence.
Background and purpose: Persistent intrathecal production of IgG...
19th July, 2008
Department of Neurology, Ulleval University Hospital, Oslo, Norway. Eur J Neurol. 2008 Jul 9;.
DOI Direct Link
5alpha-dihydrotestosterone up-regulates transthyretin levels in mice and rat choroid plexus via an androgen receptor independent pathway.
Transthyretin (TTR) is a 55 kDa plasma homotetrameric protein mainly...
19th July, 2008
Health Sciences Research Centre - CICS, University of Beira Interior, Av. Brain Res. 2008 Jul 2;.
DOI Direct Link
Refinement of a commercial bench-top relaxin assay for pregnancy diagnosis using urine from domestic and nondomestic felids.
Relaxin, a 6-kDa polypeptide hormone, is excreted in the urine during...
19th July, 2008
Center for Conservation and Research of Endangered Wildlife, Cincinnati J Zoo Wildl Med. 2008 Jun;39(2):170-9.
Hypotonicity and Peptide Discharge from a Single Vesicle.
Neuroendocrine secretory vesicles discharge their cargo in response to a...
18th July, 2008
NICHD, NIH. Am J Physiol Cell Physiol. 2008 Jul 16;.
DOI Direct Link
Temporal and spatial expression of IGF-I and IGFBP-1 during acute-phase response induced by localized inflammation in rats.
OBJECTIVE: The acute-phase response (APR), a cytokine-induced defense...
18th July, 2008
Department of Medicine, Division of Gastroenterology and Endocrinology, Growth Horm IGF Res. 2008 Jul 14;.
DOI Direct Link

View more research publications.

BioNews Results for Radioimmunoassay:

RSS Logo
BioPortfolio Logo
Key Challenges and Issues facing the World Immunoassay Instruments Market
Macro World Investor:  Jul 2 2008 5:50PM Matching: radioimmunoassay
Key Challenges and Issues facing the World Immunoassay Instruments Market
Calibre Macro World:  Jul 2 2008 5:17PM Matching: radioimmunoassay

Radioimmunoassay Clinical Trials:

Clinical Trials Logo
Effect of a Low-Calorie Diet and/or Exercise Program on Risk Factors for Developing Breast Cancer in Overweight or Obese Postmenopausal Women
Breast Cancer;   Obesity;   Weight Changes

Radioimmunoassay Patents:

USPTO Logo
US Patent No.Title
7393917 PRO1482 polypeptides
7396663 Isolated polynucleotide encoding a G-protein coupled receptor
7396691 Method for the detection of fluoride or hydrogen fluoride and detection kit
7396809 Methods and reagents for treating glucose metabolic disorders
7396810 Compositions and methods for treating cancer by modulating HER-2 and EGF receptors

View more patents.

Companies working in the area of Radioimmunoassay from Bioportfolio's Biocorporate database :

BioPortfolio Logo
Company Name

Cisbio-US, Inc

Cisbio International is a privately held innovative company committed to improving human healthcare with more than 50 years of experience in the fileds of therapy, medical diagnostic and drug...
www.htrf-assays.com/        View full profile
Thumbshot

MEDICORP LTD

MEDICORP is Canada's largest, independent ISO 9001-certified manufacturer and distributor of immunodiagnostic and microbiology products. Included are enzyme immunoassay [ELISA], immunoradiometric ...
www.medicorp.com/        View full profile
Thumbshot

BioPortfolio Ltd. offers e-mail and postal lists for Radioimmunoassay scientists - we have details of around 8290 individuals working on Radioimmunoassay .


This page has been viewed 142 times
Recent Search Terms used to find this page: radioimmunoassay | radio-immunoassay Nobel prize winner | radioimmunoassay | Radioimmunoassay | .
Browse BioPortfolio's InDepth service - alphabetically: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z or by Most Publications, recently searched for, or most viewed.

Search for Radioimmunoassay across BioPortfolio, or bestselling Radioimmunoassay books or recently published Radioimmunoassay books .
Wikipedia excerpt, where present, licenced under the GNU Free Documentation License. Resources from the NCBI applied. Selected MeSH subject headings created and maintained by the US NLM are used in conjunction with additional keywords. 2006-2008 MeSH.
 

Nothing in this website should be used in place of personal medical advice from your own qualified medical practitioner.  See User Agreement

Send comments and feedback to:
Peter Barfoot Managing Director, BioPortfolio Ltd.
UK Tel: (+44) 1300 321501
USA Voicemail and Fax: (+1) 415 680 2472

All rights reserved. All other trademarks recognized.

BioPortfolio Limited is registered in England & Wales at Wessex Barn, Dorchester Road, Frampton, Dorset, DT2 9NB, UK. No.3312883 VAT No. GB 744 6483 10

Copyright 1997-2008 - BioPortfolio Limited.

BioPortfolio logo

Valid XHTML 1.0 Transitional