Urease

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows:₂CO + H₂O → CO₂ + 2NH₃In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants.Characteristics:*Active site metal: nickel* Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease .* Optimum pH: 7.4* Optimum Temperature: 60 degrees Celsius* Enzymatic specificity: urea and hydroxyurea* Inhibitors: heavy metalsThe multi-subunit enzyme usually has a 3:3 stoichiometry with a 2-fold symmetric structure . An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits . This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise gastric acid. The presence of urease is used in the diagnosis of Helicobacter species. (From the Wikpedia article Urease.)

Download PDF containing detailed information.

Recent Publications on Urease:
Clarithromycin Resistance and Efficacy of Clarithromycin-Containing Triple Eradication Therapy for Helicobacter pylori Infection in Type 2 Diabetes Mellitus Patients. BACKGROUND:: Antimicrobial resistance in Helicobacter pylori infection is...	30th October, 2009	From the Department of Gastroenterology, Baskent University Faculty of	South Med J. 2009 Oct 6. DOI Direct Link
Effects of plant diversity on nutrient retention and enzyme activities in a full-scale constructed wetland. This study focused on the relationship between plant diversity (six...	30th October, 2009	School of Life Sciences, Taizhou University, Linhai 317000, PR China.	Bioresour Technol. 2009 Oct 26. DOI Direct Link
Influence of oxytetracycline on the structure and activity of microbial community in wheat rhizosphere soil. The microbial community composition in wheat rhizosphere was analyzed by...	30th October, 2009	College of Life Sciences, Henan Normal University, Xinxiang 453007, China.	J Environ Sci (China). 2009;21(7):954-9.
Enzyme activities and heavy metals concentration in soil amended with sewage sludge. Municipal sewage sludge (MSS) and yard waste compost (YWC) provide...	30th October, 2009	Department of Plant and Soil Science, Land Grant Program, Kentucky State	J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009
Placental Features of Chorioamnionitis Colonized with Ureaplasma Species in Preterm Delivery. Ureaplasma spp. is detected in the urogenital tract, including the vagina,...	28th October, 2009	Departments of Developmental Medicine [F.N., T.H., K.N., M.N., M.N., K.M.,	Pediatr Res. 2009 Oct 24. DOI Direct Link
→View more research publications.

Press Releases About Urease:
Catachem Announces FDA Approval of the Blood Urea Nitrogen (BUN) In-Vitro	1st January, 1970	Catachem	Catachem Announces FDA Approval of the Blood Urea Nitrogen (BUN) In-Vitro Diagnostic (IVD) Chemistry Reagent Kit Bridgeport, Connecticut, USA (June 7, 1989)…. Catachem, Inc. (Private)

Urease Clinical Trials:
Feasibility Study of Phototherapy System to Treat H Pylori

Urease Patents:
US Patent No.	Title
7582463	Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
7589167	ZA loops of bromodomains
7589180	Specific binding proteins and uses thereof
7589181	Minimally immunogenic variants of SDR-grafted humanized antibody CC49 and their use
7592003	Regulation of toll-like receptors on stem cells
→View more patents.

BioPortfolio Ltd. offers e-mail and postal lists for Urease scientists - we have details of around 688 individuals working on Urease .