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Glyco‐modulation of therapeutic proteins produced in plants has shown great success. Plant‐based expression platforms for tailored human‐like N‐glycosylation are based on the overexpression of foreign genes. However, drawbacks such as protein miss targeting, interference with endogenous glycosyltransferases or with plant development hamper the widespread use of the technology. Here we describe a technique that facilitates the generation of recombinant proteins with targeted N‐glycosylation at high homogeneity. We focused on the synthesis of human type β1,4‐galactosylation by the overexpression of the human β1,4‐galactosyltransferase (GalT) in Nicotiana benthamiana. A GalT construct that targets the enzyme to the required late Golgi compartment (STGalT) was transiently co‐expressed with two pharmaceutically relevant glycoproteins. The impact of eight promoters driving the expression of STGalT was evaluated by mass spectrometry (MS) based analyses. We show that five promoters (amongst them high expressors) induce aberrant non‐human glycosylation. In contrast, three promoters, considered as moderately active, regulate gene expression to levels leading to an improved efficiency of di‐galactosylation (and subsequent sialylation) on the reporter proteins. The results point to the importance of promoter choice for optimizing glycan engineering processes.
Original Article: Promoter choice impacts the efficiency of plant glyco‐engineeringNEXT ARTICLE
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...