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Single-Cell Genomics Advances with Development of Novel Analytical Technique

04:14 EDT 1 Aug 2017 | Genetic Engineering News

Researchers at the New York Genome Center (NYGC) say they have developed a technique that greatly improves the ability to carry out single-cell RNA sequencing. CITE-seq, or Cellular Indexing of Transcriptomes and Epitopes by sequencing, couples the measurement of surface protein markers on thousands of single cells with simultaneous sequencing of the messenger RNA (mRNA or transcriptomes) of those same single cells, according to the scientists.. The team’s methodology (“Simultaneous epitope and transcriptome measurement in single cells”), published in Nature Methods , monitored 10 surface proteins, along with transcriptomes, of 8,000 single cells. “High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels,” write the investigators. “Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome ...

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