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Intercalating Dyes or Fluorescent Probes For RT-qPCR?

03:00 EDT 3 Aug 2017 | Bitesize Bio

The unique feature of real-time quantitative polymerase chain reaction (RT-qPCR) is that it associates the amplification of your target gene with a fluorescent signal in a quantifiable manner. Presently, there are numerous fluorescent tool kits/methods to consider when designing your RT-qPCR experiment. However, the two major categories to choose from are fluorescent intercalating dyes and…

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