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Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for in vitro Neural Differentiation

21:41 EDT 8 Aug 2017 | Wiley Biotechnology Journal

The extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. In this study, we investigated the stimulation of neural differentiation by a laminin protein‐derived epitope in two‐dimensional (2D) and three‐dimensional (3D) environments. The 3D gel system was found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibited a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC‐12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro.

Original Article: Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for in vitro Neural Differentiation

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