Syndicated Content about Biotechnology, Pharmaceutical and Healthcare

18:37 EDT 1st July 2016 | BioPortfolio

Latest global life science, pharmaceutical and healthcare news syndicated from over 50 publishers. 

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Friday 1st July 2016

Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching

MicroRNA-155 induces AML in combination with the loss of C/EBPA in mice

Patient-derived xenotransplants can recapitulate the genetic driver landscape of acute leukemias

Subtype-specific patterns of molecular mutations in acute myeloid leukemia

Identification of blood vascular endothelial stem cells by the expression of protein C receptor

A high-resolution transcriptome map of cell cycle reveals novel connections between periodic genes and cancer

Distribution of IgG galactosylation as a promising biomarker for cancer screening in multiple cancer types

Diagnostic and predictive biomarkers for lymphoma diagnosis and treatment in the era of precision medicine

Standardized evaluation of tumor-infiltrating lymphocytes in breast cancer: results of the ring studies of the international immuno-oncology biomarker working group

Exploring chromosomal abnormalities and genetic changes in uterine smooth muscle tumors

Integrated fabrication‐conjugation methods for polymeric and hybrid microparticles for programmable drug delivery and biosensing applications

Functionalized polymeric microparticles possess significant potential for controlled drug delivery and biosensing applications, yet current fabrication techniques face challenges in simple and scalable fabrication and biofunctionalization. For programmable manufacture of biofunctional microparticles in a simple manner, we have developed robust micromolding methods combined with biopolymeric conjug...

Enabling xylose utilization in Yarrowia lipolytica for lipid production

The conversion of lignocellulosic sugars, in particular xylose, is important for sustainable fuels and chemicals production. While the oleaginous yeast Yarrowia lipolytica is a strong candidate for lipid production, it is currently unable to effectively utilize xylose. By introducing a heterologous oxidoreductase pathway and enabling starvation adaptation, we obtained a Y. lipolytica strain, E26 X...

Facile Construction of Random Gene Mutagenesis Library for Directed Evolution Without the Use of Restriction Enzyme in Escherichia coli

A foolproof protocol was developed for the construction of mutant DNA library for directed protein evolution. First, a library of linear mutant gene was generated by error‐prone PCR or molecular shuffling, and a linear vector backbone was prepared by high‐fidelity PCR. Second, the amplified insert and vector fragments were assembled by overlap‐extension PCR with a pair of 5'‐phosphorylated...

Mixing at the Microscale: Power Input in Shaken Microtiter Plates

Power input and local energy dissipation are crucial parameters for the engineering characterization of mixing and fluid dynamics at the microscale. Since hydrodynamic stress is solely dependent on the maximum power input, we adapted the clay/polymer method to obtain flock destruction kinetics in 6‐, 24‐, and 96‐well microtiter plates on orbital shakers. We also determined the specific power...

The Ssr protein (T1E_1405) from Pseudomonas putida DOT‐T1E enables oligonucleotide‐based recombineering in platform strain P. putida EM42

Some strains of the soil bacterium Pseudomonas putida have become in recent years platforms of choice for hosting biotransformations of industrial interest. Despite availability of many genetic tools for this microorganism, genomic editing of the cell factory P. putida EM42 (a derivative of reference strain KT2440) is still a time‐consuming endeavor. In this work we have investigated the in vivo...

Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly

Nap1 is a histone chaperone involved in the nuclear import of H2A–H2B and nucleosome assembly. Here, we report the crystal structure of Nap1 bound to H2A–H2B together with in vitro and in vivo functional studies that elucidate the principles underlying Nap1-mediated H2A–H2B chaperoning and nucleosome assembly. A Nap1 dimer provides an acidic binding surface and asymmetr...

Histone H3.3 promotes IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA

Immunoglobulin diversification is driven by activation-induced deaminase (AID), which converts cytidine to uracil within the Ig variable (IgV) regions. Central to the recruitment of AID to the IgV genes are factors that regulate the generation of single-stranded DNA (ssDNA), the enzymatic substrate of AID. Here, we report that chicken DT40 cells lacking variant histone H3.3 exhibit reduced IgV seq...

WRNIP1 protects stalled forks from degradation and promotes fork restart after replication stress

Accurate handling of stalled replication forks is crucial for the maintenance of genome stability. RAD51 defends stalled replication forks from nucleolytic attack, which otherwise can threaten genome stability. However, the identity of other factors that can collaborate with RAD51 in this task is poorly elucidated. Here, we establish that human Werner helicase interacting protein 1 (WRNIP1) is loc...

Novel function of Tau in regulating the effects of external stimuli on adult hippocampal neurogenesis

Tau is a microtubule-associated neuronal protein found mainly in axons. However, its presence in dendrites and dendritic spines is particularly relevant due to its involvement in synaptic plasticity and neurodegeneration. Here, we show that Tau plays a novel in vivo role in the morphological and synaptic maturation of newborn hippocampal granule neurons under basal conditions. Furthermore, we...

SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development

Skp1-Cul1-F-box protein (SCF) ubiquitin ligases direct cell survival decisions by controlling protein ubiquitylation and degradation. Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Although Sufu has a pivotal role in Hh signaling, the players invo...

HSV-1 ICP27 targets the TBK1-activated STING signalsome to inhibit virus-induced type I IFN expression

Herpes simplex virus (HSV) 1 stimulates type I IFN expression through the cGAS–STING–TBK1 signaling axis. Macrophages have recently been proposed to be an essential source of IFN during viral infection. However, it is not known how HSV-1 inhibits IFN expression in this cell type. Here, we show that HSV-1 inhibits type I IFN induction through the cGAS–STING–TBK1 pathway in h...

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions

In hypoxic cells, dysfunctional mitochondria are selectively removed by a specialized autophagic process called mitophagy. The ER–mitochondrial contact site (MAM) is essential for fission of mitochondria prior to engulfment, and the outer mitochondrial membrane protein FUNDC1 interacts with LC3 to recruit autophagosomes, but the mechanisms integrating these processes are poorly understood. H...

Mitochondria just wanna have FUN(DC1)

A fascinating story is unfolding at the interface between mitochondria and the ER. Two new papers, one in this issue of The EMBO Journal (Wu et al, 2016) and one in the journal Autophagy (Chen et al, 2016), further clarify the role of mitochondrial outer membrane protein FUNDC1 in autophagy and connect it to mitochondrial fission occurring at the interface between mitochondria and the ER...

Cryoport enhances cold chain tracking with SmartPack II

Saying that his company is preparing for future business in cellular therapy and related live-cell treatments, even ahead of a commercial market, Jerrell Shelton, president of Cryoport, is promoting the company’s newly introduced SmartPack II condition-monitoring system. The electronic monitoring […] The post Cryoport enhances cold chain tracking with SmartPack II appeared first on Pharmac...

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