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A Guide to Green-Fluorescent Protein
Click to purchase:
A Guide to Green-Fluorescent Protein
200+ Pages, 45+ Exhibits, Sample checklist of questions for GFP variant
evaluation
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This Guide seeks to describe
Green-Fluorescent Protein's usefulness in biomedical programs and
applications.
Proteomics and genomics have provided a rich supply of targets in
drug discovery programs. However, selecting and understanding both
drugs and targets in the context of the living cell are critical to
early evaluation of drug action and efficacy. This challenge has
given rise to the development of cellular and molecular probes of
drug action and a new emphasis on 'cellomics.'
One probe gaining in popularity is the Green Fluorescent Protein
(GFP). As a fluorescent probe, its rare genetic encoding enables
both real-time and non-invasive reporting in living cells. This
ability provides a basis for cell-based monitoring of GFP-linked
targets upon administration of external drugs. The impact of GFP has
been revolutionary. GFP has not just facilitated visualization of
intricate cellular architecture but it has also acted as a marker of
protein dynamics and behavior in cell biology. These applications
have been translated to drug discovery where, GFP, and its color
variants have been utilized in fluorescence and confocal imaging,
HTS/HCS screening assays and in in vivo diagnostics. GFP can not
only be used in early stage target characterization but also in
retrieving non-invasive 'whole organism' data and in evaluating lead
compound toxicology.
This Guide will:
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Demonstrate the value of GFP applications in cell biology-which
have accelerated GFP implementation into drug discovery programs
that relate to living cells.
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Illustrate how GFP is currently being used in the
biopharmaceutical industry.
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Evaluate many of the available GFP and 'GFP-like' variants and
present their strengths and weaknesses.
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Review the instruments suitable for GFP detection.
Get D&MD's Guide to Green-Fluorescent Protein today to
learn about why and how GFP is being used in biopharma and it's
suitability in drug discovery applications. |
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This Guide will Prove Beneficial to:
Directors of Research & Development and Business Development at
Pharmaceutical, Biotechnology, CRO's, and Diagnostic companies.
In addition, this Guide will prove invaluable to
Group Leaders, Senior Scientists, Post-Doctoral and Technical
Researchers within Research Institutions, Government Research
Institutes (biology), and Universities. Also expected to benefit are
Directors of Research & Development and Business Development within
companies that provide molecular and drug discovery tools and
fluorescent detection instruments. This Guide could also be useful
for Educators, Instructors, and Lecturers who provide GFP/biotechnology
workshops and continuing education courses.
This Guide presents an overview of the most
important features of Green-Fluorescent Protein (GFP) and is an
invaluable, practical resource for:
Those not currently using GFP it will serve as a
valuable introduction to the potential uses of Green-Fluorescent
Protein.
Those using GFP it explains how to maximize data
acquisition and data interpretation.
Those interested in cloning, expressing and purifying
GFP it provides some useful considerations and pointers.
This Guide will answer the following questions:
What questions need to be asked when evaluating a new
GFP variant?
What measures can be taken in creating a successful
GFP fusion?
What are the common problems encountered in
monitoring GFP fluorescence?
Where does GFP fit in to drug discovery?
How can I use GFP in multicolor labeling experiments?
How can I avoid false positives or false negatives in
GFP-based assays?
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Table of Contents |
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Section |
Summary |
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1 |
Executive Summary
1.1 Purpose and scope
1.2 Summary
1.3 Additional information |
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2 |
Introduction
2.1 Historical perspective
2.2 GFP fundamentals
2.3 Cell biology applications
2.4 Drug discovery applications
2.5 Definitions |
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3 |
GFP Fundamentals
3.1 General biology of Aequorea victoria and GFP
3.2 Molecular biology of GFP
3.3 Chemical reagents and factors which affect GFP stability
3.4 Physical characteristics of GFP fluorescent
3.5 Common problems in GFP fluorescent detection |
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4 |
GFP Variant Evaluation
4.1 Parameters to consider when evaluating GFP variants
4.2 Characterized GFP and 'GFP-like' variants
4.3 Available crystallography data
4.4 Sample checklist of questions for GFP variant evaluation |
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5 |
GFP Gene Cloning and Expression
5.1 Commercially available GFP cloning vectors
5.2 Arranging and re-arranging GFP clone architecture
5.3 GFP fusion proteins
5.4 GFP fusion partners
5.5 GFP gene delivery
5.6 GFP licenses
5.7 GFP gene expression |
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6 |
GFP Protein Purification
6.1 Traditional preparative purification methods
6.2 Organic extraction of GFP
6.3 Analytical protein purification
6.4 High throughput protein purification |
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7 |
Fluorescence Detection and GFP
7.1 General considerations
7.2 Spectrophotometry
7.3 Fluorimetry
7.4 Fluorometric plate readers
7.5 Flow cytometry
7.6 Native PAGE electrophoresis
7.7 Fluorescence microscopy and imaging |
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8 |
Calibrations and Data Analysis
8.1 Data output from spectrophotometers
8.2 Data output from fluorimeters
8.3 Data output from fluorimetric plate readers
8.4 Data output from flow cytometry
8.5 Data output from microscopy
8.6 Data output from imaging |
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GFP Applications in Cell Biology
9.1 GFP at various levels of cell biology
9.2 Strategies for monitoring GFP in cell biology
9.3 Advantages of using GFP in cell biology
9.4 Limitations of using GFP in cell biology
9.5 Experimental considerations |
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10 |
GFP Applications in Drug Discovery
10.1 Assay detection modes and drug discovery
10.2 Drug discovery process
10.3 Specific applications of GFP in drug discovery |
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11 |
GFP as an Educational Tool in Biotechnology
11.1 GFP as an educational tool
11.2 Workshops
11.3 Biotechnology educational kits |
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12 |
Emerging Technologies
12.1 New GFP variants
12.2 Developments in model systems
12.3 Developments in detection instruments
12.4 Array technology
12.5 Developments in drug discovery |
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13 |
Appendices
13.1 Vendors
13.2 GFP-related books
13.3 Relevant journals
13.4 Societies
13.5 Symposia
13.6 Online protocols
13.7 GFP-related web-sites
13.8 Selected media reports
13.9 GFP fact sheet |
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14 |
References
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Price Hard Copy
(US$):
$395.00
Interested in this
report:
peter.barfoot@bioportfolio.com
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