Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISA.
Summary of "Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISA."
Geotrichum candidum is a common soil-borne fungus that causes sour-rot of tomatoes, citrus fruits and vegetables, and is a major contaminant on tomato processing equipment. The aim of this work was to produce a monoclonal antibody and diagnostic assay for its detection in tomato fruit and juice. Using hybridoma technology, a cell line (FE10) was generated that produced a monoclonal antibody belonging to the immunoglobulin class M (IgM) that was specific to G. candidum and the closely related teleomorphic species Galactomyces geotrichum and anamorphic species Geotrichum europaeum and Geotrichum pseudocandidum in the G. geotrichum/G. candidum complex. The MAb did not cross-react with a wide range of unrelated fungi, including some likely to be encountered during crop production and processing. The MAb binds to an immunodominant high molecular mass (> 200 kDa) extracellular polysaccharide antigen that is present on the surface of arthroconidia and hyphae of G. candidum. The MAb was used in a highly specific enzyme-linked immunosorbent assay (ELISA) to accurately detect the fungus in infected tomato fruit and juice. Specificity of the ELISA was confirmed by sequencing of the internally transcribed spacer (ITS) 1-5.8S-ITS2 rRNA-encoding regions of fungi isolated from naturally-infected tomatoes.
University of Exeter, Exeter, United Kingdom. C.R.Thornton@ex.ac.uk
This article was published in the following journal.
Name: International journal of food microbiology
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20850192
- DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2010.08.012
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Medical and Biotech [MESH] Definitions
A plant genus of the family FABACEAE known for its sour fruit.
A plant species of the family VACCINIUM known for the sour fruit which is sometimes used for urinary tract infections.
Protein-digesting and milk-clotting enzymes found in PINEAPPLE fruit juice and stem tissue. Enzymes from the two sources are distinguished as fruit bromelain and stem bromelain. There has been interest in using it for INFLAMMATION and DEBRIDEMENT. This enzyme was formerly listed as EC 22.214.171.124.
Fermented juice of fresh grapes or of other fruit or plant products used as a beverage.
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.