Identification of glycolaldehyde as the key inhibitor of bioethanol fermentation by yeast and genome-wide analysis of its toxicity.

00:22 EST 28th February 2015 | BioPortfolio

Summary of "Identification of glycolaldehyde as the key inhibitor of bioethanol fermentation by yeast and genome-wide analysis of its toxicity."

Degradation of lignocellulose with pressurised hot water is an efficient method of bioethanol production. However, the resultant solution inhibits ethanol fermentation by Saccharomyces cerevisiae. Here, we first report that glycolaldehyde, which is formed when lignocellulose is treated with pressurised hot water, inhibits ethanol fermentation. The final concentration of glycolaldehyde formed by the treatment of lignocellulose with pressurised hot water ranges from 1 to 24 mM, and 1-10 mM glycolaldehyde was sufficient to inhibit fermentation. This result indicates that glycolaldehyde is one of the main substances responsible for inhibiting fermentation after pressurised hot water degradation of lignocellulose. Genome-wide screening of S. cerevisiae revealed that genes encoding alcohol dehydrogenase, methylglyoxal reductase, polysomes, and the ubiquitin ligase complex are required for glycolaldehyde tolerance. These novel findings will provide new perspectives on breeding yeast for bioethanol production from biomass treated with pressurised hot water.

Affiliation

Department of Environmental Sciences, Faculty of Agriculture, Saga University, Saga, 840-8502, Japan.

Journal Details

This article was published in the following journal.

Name: Biotechnology letters
ISSN: 1573-6776
Pages:

Links

PubMed Articles [16040 Associated PubMed Articles listed on BioPortfolio]

Comparison of γ-irradiation with other pretreatments followed with simultaneous saccharification and fermentation on bioconversion of microcrystalline cellulose for bioethanol production.

The effect of γ-irradiation pretreatment was compared with other pretreatment methods including ionic liquids (ILs), 1% HCl, 1% H2SO4, acidic aqueous Ils (AA-ILs), on the bioconversion efficiency of ...

Amphipathic lignin derivatives to accelerate simultaneous saccharification and fermentation of unbleached softwood pulp for bioethanol production.

Amphipathic lignin derivatives (A-LDs) were already demonstrated to improve enzymatic saccharification of lignocellulose. Based on this knowledge, two kinds of A-LDs prepared from black liquor of soda...

Bioethanol Production from Fermentable Sugar Juice.

Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing ...

Genome-wide association study success in ophthalmology.

Much progress in our understanding of the genetic profile of many ophthalmic diseases has been made over the last decade. Identification of novel gene associations allows insight into the mechanisms o...

High temperature simultaneous saccharification and fermentation of starch from inedible wild cassava (Manihot glaziovii) to bioethanol using Caloramator boliviensis.

The thermoanaerobe, Caloramator boliviensis was used to ferment starch hydrolysate from inedible wild cassava to ethanol at 60°C. A raw starch degrading α-amylase was used to hydrolyse the cassava s...

Clinical Trials [1782 Associated Clinical Trials listed on BioPortfolio]

Analysis of Genomic DNA Alterations in Familial Schizophrenia

Persons with schizophrenia experience imaginary voices, visions and disorganized thoughts, and are handicapped when it comes to social life, which is detrimental to the affected individual...

Development of Escitalopram Genomic Device by Using Candidate Gene Approach and Genome-Wide Scanning

To reveal the genetic determinants of the treatment outcome of escitalopram in depressed patients (by using candidate gene approach and whole genome scanning).

Mitral Valve Prolapse (MVP) - France Study

This prospective nation-wide (France) study aims to search for susceptibility genes in MVP using a genome wide analysis and comparing results obtained in 1000 patients with MVP and 1000 n...

Effects of Supplement Containing Various Probiotics and Fermentation Products on Obesity

The purpose of this study is to determine the effect of supplement combined various probiotics and fermentation products on body mass index, body fat mass, abdominal circumference, viscera...

Safety of Red Yeast Rice for High Cholesterol in Individuals With Statin Intolerance

This study will examine the effect of red yeast rice extract compared to pravastatin on muscle related complaints in individuals with high cholesterol who have previously been unable to to...

Medical and Biotech [MESH] Definitions

An enzyme that catalyzes the oxidation of glycolaldehyde to glycolate, using NAD and NADP as COENZYMES.

A genus of the ascomycetous yeast in the family Saccharomycodaceae, order SACCHAROMYCETALES, that contributes to the spontaneous fermentation of cider. Anamorphic forms are in the genus KLOECKERA.

An analysis comparing the allele frequencies of all available (or a whole GENOME representative set of) polymorphic markers in unrelated patients with a specific symptom or disease condition, and those of healthy controls to identify markers associated with a specific disease or condition.

Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.

Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.

Search BioPortfolio:
Loading
Advertisement

Relevant Topic

Bioinformatics
Latest News Clinical Trials Research Drugs Reports Corporate
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Advertisement

Searches Linking to this Article