Advertisement

Topics

Identification of glycolaldehyde as the key inhibitor of bioethanol fermentation by yeast and genome-wide analysis of its toxicity.

Summary of "Identification of glycolaldehyde as the key inhibitor of bioethanol fermentation by yeast and genome-wide analysis of its toxicity."

Degradation of lignocellulose with pressurised hot water is an efficient method of bioethanol production. However, the resultant solution inhibits ethanol fermentation by Saccharomyces cerevisiae. Here, we first report that glycolaldehyde, which is formed when lignocellulose is treated with pressurised hot water, inhibits ethanol fermentation. The final concentration of glycolaldehyde formed by the treatment of lignocellulose with pressurised hot water ranges from 1 to 24 mM, and 1-10 mM glycolaldehyde was sufficient to inhibit fermentation. This result indicates that glycolaldehyde is one of the main substances responsible for inhibiting fermentation after pressurised hot water degradation of lignocellulose. Genome-wide screening of S. cerevisiae revealed that genes encoding alcohol dehydrogenase, methylglyoxal reductase, polysomes, and the ubiquitin ligase complex are required for glycolaldehyde tolerance. These novel findings will provide new perspectives on breeding yeast for bioethanol production from biomass treated with pressurised hot water.

Affiliation

Department of Environmental Sciences, Faculty of Agriculture, Saga University, Saga, 840-8502, Japan.

Journal Details

This article was published in the following journal.

Name: Biotechnology letters
ISSN: 1573-6776
Pages:

Links

DeepDyve research library

PubMed Articles [16433 Associated PubMed Articles listed on BioPortfolio]

Polygenic analysis and targeted improvement of the complex trait of high acetic acid tolerance in the yeast Saccharomyces cerevisiae.

Acetic acid is one of the major inhibitors in lignocellulose hydrolysates used for the production of second-generation bioethanol. Although several genes have been identified in laboratory yeast strai...

Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre.

This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210°C, 10min) and then subjected to simultaneous saccharification and ...

Molecular characterization of HIV-1 genome in fission yeast Schizosaccharomyces pombe.

The human immunodeficiency virus type 1 (HIV-1) genome (~9 kb RNA) is flanked by two long terminal repeats (LTR) promoter regions with nine open reading frames, which encode Gag, Pol and Env polyprot...

Bioethanol production from carbohydrate-enriched residual biomass obtained after lipid extraction of Chlorella sp. KR-1.

The residual biomass of Chlorella sp. KR-1 obtained after lipid extraction was used for saccharification and bioethanol production. The carbohydrate was saccharified using simple enzymatic and chemica...

Generation of bioethanol and VFA through anaerobic acidogenic fermentation route with press mud obtained from sugar mill as a feedstock.

Acidogenic anaerobic fermentation route was explored for the production of bioethanol and volatile fatty acids (VFA) from the press mud (PM) obtained from sugar mill. Slurry was prepared from PM havin...

Clinical Trials [2412 Associated Clinical Trials listed on BioPortfolio]

Analysis of Genomic DNA Alterations in Familial Schizophrenia

Persons with schizophrenia experience imaginary voices, visions and disorganized thoughts, and are handicapped when it comes to social life, which is detrimental to the affected individual...

Lipid-lowering Effect of Phytosterols, Red Yeast Rice and Their Combination

A large body of evidence confirm the cholesterol lowering effect of phytosterols and red yeast rice. Because their mechanisms of action mime the ones of chemical statins and cholesterol ab...

Development of Escitalopram Genomic Device by Using Candidate Gene Approach and Genome-Wide Scanning

To reveal the genetic determinants of the treatment outcome of escitalopram in depressed patients (by using candidate gene approach and whole genome scanning).

Mitral Valve Prolapse (MVP) - France Study

This prospective nation-wide (France) study aims to search for susceptibility genes in MVP using a genome wide analysis and comparing results obtained in 1000 patients with MVP and 1000 n...

Effects of Supplement Containing Various Probiotics and Fermentation Products on Obesity

The purpose of this study is to determine the effect of supplement combined various probiotics and fermentation products on body mass index, body fat mass, abdominal circumference, viscera...

Medical and Biotech [MESH] Definitions

An enzyme that catalyzes the oxidation of glycolaldehyde to glycolate, using NAD and NADP as COENZYMES.

A genus of the ascomycetous yeast in the family Saccharomycodaceae, order SACCHAROMYCETALES, that contributes to the spontaneous fermentation of cider. Anamorphic forms are in the genus KLOECKERA.

An analysis comparing the allele frequencies of all available (or a whole GENOME representative set of) polymorphic markers in unrelated patients with a specific symptom or disease condition, and those of healthy controls to identify markers associated with a specific disease or condition.

Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.

Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.

Quick Search
Advertisement
 


DeepDyve research library

Relevant Topic

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


Searches Linking to this Article