A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria.
Summary of "A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria."
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
Affiliation
Department of Microbiology, Kyungpook National University, School of Medicine, Daegu, 700-422, Republic of Korea.
Journal Details
This article was published in the following journal.
Name: Journal of microbiology (Seoul, Korea)
ISSN: 1976-3794
Pages: 709-11
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21046353
- DOI: http://dx.doi.org/10.1007/s12275-010-0299-z
Medical and Biotech [MESH] Definitions
Disk Diffusion Antimicrobial Tests
A method where a culturing surface inoculated with microbe is exposed to small disks containing known amounts of a chemical agent resulting in a zone of inhibition (usually in millimeters) of growth of the microbe corresponding to the susceptibility of the strain to the agent.
Serum Bactericidal Test
Method of measuring the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy. It is used to monitor the therapy in BACTERIAL ENDOCARDITIS; OSTEOMYELITIS and other serious bacterial infections. As commonly performed, the test is a variation of the broth dilution test. This test needs to be distinguished from testing of the naturally occurring BLOOD BACTERICIDAL ACTIVITY.
Colony Count, Microbial
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. Each colony (i.e., microbial colony-forming unit) represents the progeny of a single cell in the original inoculum. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Bacteriophage Typing
A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.
Embryo Culture Techniques
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS
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