Stamp wound assay for studying coupled cell migration and cell debris clearance.
Summary of "Stamp wound assay for studying coupled cell migration and cell debris clearance."
A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence of cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro.
Affiliation
Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611, United States.
Journal Details
This article was published in the following journal.
Name: Langmuir : the ACS journal of surfaces and colloids
ISSN: 1520-5827
Pages: 16672-6
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20961056
- DOI: http://dx.doi.org/10.1021/la103542y
Medical and Biotech [MESH] Definitions
Cell Migration Inhibition
Phenomenon of cell-mediated immunity measured by in vitro inhibition of the migration or phagocytosis of antigen-stimulated LEUKOCYTES or MACROPHAGES. Specific CELL MIGRATION ASSAYS have been developed to estimate levels of migration inhibitory factors, immune reactivity against tumor-associated antigens, and immunosuppressive effects of infectious microorganisms.
Comet Assay
A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.
Cell-in-cell Formation
The mechanisms by which a cell becomes internalized in another. The host cell may engulf another as do PHAGOCYTIC CELLS, or the host cell may be invaded by another cell (ENTOSIS), or internalization processes may involve the cooperation of both the host cell and the cell being internalized. Viable cells may remain in non-phagocytic cells (EMPERIPOLESIS), undergo cell division, pass through and then out of the host cell (TRANSCELLULAR CELL MIGRATION), or trigger APOPTOSIS of the invaded cell.
Cell Migration Assays, Macrophage
Assays that measure the rate of migration of MACROPHAGES. They may involve the use hollow plastic chamber, sealed at one end with a porous membrane and suspended over a larger well which may contain CHEMOTACTIC FACTORS. The migration of cell through the pores to the other side of the membrane is measured.
Cell Migration Assays
Specific assays that measure the migration of cells. They are commonly used to measure the migration of immune cells in response to stimuli and the inhibition of immune cell migration by immunosuppressive factors.
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