Applicability of a yeast bioassay in the detection of steroid esters in hair.
Summary of "Applicability of a yeast bioassay in the detection of steroid esters in hair."
The aim of the present study was to demonstrate the applicability of a yeast androgen and estrogen bioassay in the detection of steroid esters in hair samples of animals treated with a hormone ester cocktail. The outcome of the activity screenings was critically compared with the results previously obtained with LC-MS/MS analysis. Hair samples of one pour-on treated animal, 10 ml DMSO containing 25 mg estradiol benzoate (EB), 60 mg testosterone decanoate (TD) and 60 mg testosterone cypionate (TC), were selected and analyzed with the androgen and estrogen yeast bioassay. Results showed that by the introduction of a hydrolysis step, bioassays can be used to screen for the presence of hormone esters in hair samples. Based on the difference in fluorescence responses between the non-hydrolyzed and the hydrolyzed hair samples, it was possible to detect the presence of EB up to at least 56 days after a single pour-on treatment and to detect the presence of TC and TD up to at least 14 days after the treatment. Although the LC-MS/MS analysis could detect TC and TD up to 49 days after treatment, bioassays have the advantage that they can also detect any (un)known steroid ester.
Affiliation
Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium, I.Becue@UGent.be.
Journal Details
This article was published in the following journal.
Name: Analytical and bioanalytical chemistry
ISSN: 1618-2650
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21079924
- DOI: http://dx.doi.org/10.1007/s00216-010-4401-5
Medical and Biotech [MESH] Definitions
Receptors, Steroid
Proteins found usually in the cytoplasm or nucleus that specifically bind steroid hormones and trigger changes influencing the behavior of cells. The steroid receptor-steroid hormone complex regulates the transcription of specific genes.
Killer Factors, Yeast
Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.
Substance Abuse Detection
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
Hair Preparations
Hair grooming and cleansing aids or other products meant for topical application to hair, usually human. They include sprays, bleaches, conditioners, rinses, shampoos, nutrient lotions, etc.
Pregnenolone
A 21-carbon steroid, derived from CHOLESTEROL and found in steroid hormone-producing tissues. Pregnenolone is the precursor to GONADAL STEROID HORMONES and the adrenal CORTICOSTEROIDS.
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