Applicability of a yeast bioassay in the detection of steroid esters in hair.

09:57 EDT 30th August 2015 | BioPortfolio

Summary of "Applicability of a yeast bioassay in the detection of steroid esters in hair."

The aim of the present study was to demonstrate the applicability of a yeast androgen and estrogen bioassay in the detection of steroid esters in hair samples of animals treated with a hormone ester cocktail. The outcome of the activity screenings was critically compared with the results previously obtained with LC-MS/MS analysis. Hair samples of one pour-on treated animal, 10 ml DMSO containing 25 mg estradiol benzoate (EB), 60 mg testosterone decanoate (TD) and 60 mg testosterone cypionate (TC), were selected and analyzed with the androgen and estrogen yeast bioassay. Results showed that by the introduction of a hydrolysis step, bioassays can be used to screen for the presence of hormone esters in hair samples. Based on the difference in fluorescence responses between the non-hydrolyzed and the hydrolyzed hair samples, it was possible to detect the presence of EB up to at least 56 days after a single pour-on treatment and to detect the presence of TC and TD up to at least 14 days after the treatment. Although the LC-MS/MS analysis could detect TC and TD up to 49 days after treatment, bioassays have the advantage that they can also detect any (un)known steroid ester.

Affiliation

Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium, I.Becue@UGent.be.

Journal Details

This article was published in the following journal.

Name: Analytical and bioanalytical chemistry
ISSN: 1618-2650
Pages:

Links

PubMed Articles [9079 Associated PubMed Articles listed on BioPortfolio]

A quick method for determination of psychoactive agents in serum and hair by using capillary electrophoresis and mass spectrometry.

The aim of the research was to develop a new sensitive method for simultaneously the determination of psychoactive drugs: 1-benzylpiperazine, 7-aminoclonazepam, alprazolam, clonazepam, diazepam, estaz...

Determinants of hair cortisol and hair cortisone concentrations in adults.

The analysis of hair cortisol concentrations (HairF) is a promising new tool for the assessment of long-term cortisol. With the development of multiple steroid analyses by means of liquid chromatograp...

A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins.

The design and synthesis of a fluorescent sensor of diphosphate esters along with its application for in-gel detection is described. Dinuclear zinc complex 1 selectively detects diphosphate esters in ...

Determination of ethyl glucuronide in hair for detection of alcohol consumption in patients after liver transplantation.

Early detection of alcohol misuse in OLT recipients is essential to offer patients support and prevent organ damage. Here, ethyl glucuronide, a metabolite of ethanol found in hair (hEtG) was evaluated...

Comparison of Next Generation Sequencing vs. Biological Indexing for the optimal detection of viral pathogens in Grapevine.

A bioassay is routinely used to determine the viral phytosanitary status of commercial grapevine propagation material in many countries around the world. That test is based on the symptoms developed i...

Clinical Trials [1314 Associated Clinical Trials listed on BioPortfolio]

The Effect of Laser Hair Removal on Permanent Hair Reduction

The purpose of this study is to learn more about how hair removal with lasers achieves, what appears to be, permanent hair reduction. Laser hair removal has become one of the most popular...

Evaluation of Scalp and Hair Shaft With Biologic Markers in African American and Caucasian Hair

The purpose of this research study is to better understand African American hair and scalp, its biologic appearance and how this relates to African Americans' perceptions of their hair and...

Safety of Red Yeast Rice for High Cholesterol in Individuals With Statin Intolerance

This study will examine the effect of red yeast rice extract compared to pravastatin on muscle related complaints in individuals with high cholesterol who have previously been unable to to...

Influence of Selenium on Prostate Cancer Related Biomarkers

Penn State Milton S. Hershey Medical Center researchers are trying to compare the effects of two different forms of selenium (selenium yeast and selenomethionine) on blood selenium levels ...

Genetic Analysis for Predicting of Relapse During Steroid Treatment for Autoimmune Pancreatitis (AIP)

To determine whether certain alleles or haplotypes of major histocompatibility complex gene are associated with AIP in Korean population, we undertook this study with high-resolution typin...

Medical and Biotech [MESH] Definitions

Proteins found usually in the cytoplasm or nucleus that specifically bind steroid hormones and trigger changes influencing the behavior of cells. The steroid receptor-steroid hormone complex regulates the transcription of specific genes.

Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

Hair grooming and cleansing aids or other products meant for topical application to hair, usually human. They include sprays, bleaches, conditioners, rinses, shampoos, nutrient lotions, etc.

A 21-carbon steroid, derived from CHOLESTEROL and found in steroid hormone-producing tissues. Pregnenolone is the precursor to GONADAL STEROID HORMONES and the adrenal CORTICOSTEROIDS.


Advertisement
 

Relevant Topics

Endocrine Disorders
Latest News Clinical Trials Research Drugs Reports Corporate
Endocrine disorders are grouped into two categories: hormone imbalance - when a gland produces too much or too little of an endocrine hormone development of lesions (such as nodules or tumors) in the endocrine system, which may or may not affect...

Endocrinology
Latest News Clinical Trials Research Drugs Reports Corporate
Diabetes Diabetes Endocrine Obesity Oxycontin Renal Disease Thyroid Disorders Endocrinology is the study of the endocrine glands and the hormones that they secrete (Oxford Medical Dictionary). There are several groups of h...

Advertisement
 

Searches Linking to this Article