Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures.
Summary of "Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures."
It is well known that fish have stronger tolerance than mammals to microcystin (MC) exposure, and such a difference is attributed to their different core body temperatures. However, no in vivo study has been conducted to investigate the effects of temperature on MC-induced toxicity in fish, a typical poikilotherm. Tolerance and detoxification response of zebrafish treated with MC-LR were investigated under three temperatures. The LD(50) values evidently increased with a decline of the temperature (547, 260 and 176 µg kg(-1) at 12, 22 and 32 °C, respectively), indicating stronger tolerance of the fish at lower temperatures. Changes in the transcription of glutathione S-transferase (GST) isoforms in the fish were observed, and their sensitivity of response in the transcription of GST mRNA was on the order of 12 > 32 > 22°C. We screened out several GST genes which were more delicate to solve the MC-LR exposure at different temperatures, i.e. GST rho1, al, p1 and theta1 in the 12 °C group, and GST zeta1 and p2 in the 22 and 32 °C groups. Our findings partly validate the hypothesis that high temperature enhances toxic effects of MCs on poikilotherms. Our studies also indicate that temperature-dependent toxic effects should be taken into account for field toxic assessment of microcystins in fish. Copyright © 2010 John Wiley & Sons, Ltd.
Fisheries College of Huazhong Agricultural University; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, People's Republic of China.
This article was published in the following journal.
Name: Journal of applied toxicology : JAT
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Medical and Biotech [MESH] Definitions
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
Studies designed to examine associations, commonly, hypothesized causal relations. They are usually concerned with identifying or measuring the effects of risk factors or exposures. The common types of analytic study are CASE-CONTROL STUDIES; COHORT STUDIES; and CROSS-SECTIONAL STUDIES.
An application that must be submitted to a regulatory agency (the FDA in the United States) before a drug can be studied in humans. This application includes results of previous experiments; how, where, and by whom the new studies will be conducted; the chemical structure of the compound; how it is thought to work in the body; any toxic effects found in animal studies; and how the compound is manufactured. (From the "New Medicines in Development" Series produced by the Pharmaceutical Manufacturers Association and published irregularly.)
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.