In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa.
Summary of "In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa."
The in vitro hepatoprotective effect of the methanolic extract from Ficus gnaphalocarpa (Miq.) Steud. ex A. Rich (Moraceae) on the CCl(4)-induced liver cell damage as well as the possible antioxidant mechanisms involved in this protective effect, were investigated. The phytochemical investigation of this methanolic extract led to the isolation of six compounds identified as: betulinic acid (1); 3-methoxyquercetin (2); catechin (3); epicatechin (4); quercetin (5); and quercitrin (6). The hepatoprotective activity of these compounds was tested in vitro against CCl(4)-induced damage in rat hepatoma cells. In addition, radical-scavenging activity, β-carotene-linoleic acid model system, ferric-reducing antioxidant parameter and microsomal lipid peroxidation assays were used to measure antioxidant activity of crude extract and isolated compounds. Silymarin and trolox were used as standard references and, respectively, exhibited significant hepatoprotective and antioxidant activities. (5), (6) and (2) showed significant antioxidant and hepatoprotective activities as indicated by their ability to prevent liver cell death and lactate dehydrogenase leakage during CCl(4) intoxication. These results suggest that the protective effects of crude extract of F. gnaphalocarpa against the CCl(4)-induced hepatotoxicity possibly involve the antioxidant effect of these compounds.
Affiliation
Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaoundé, Cameroon, donfackhubert@yahoo.fr.
Journal Details
This article was published in the following journal.
Name: Inflammopharmacology
ISSN: 1568-5608
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21088994
- DOI: http://dx.doi.org/10.1007/s10787-010-0070-4
Medical and Biotech [MESH] Definitions
Picrorhiza
A plant genus of the family SCROPHULARIACEAE. Members contain scrosides (CINNAMATES) and phenylethanoid glycoside. P. kurrooa is the source of picroliv (a purified iridoid glycoside fraction from the roots having hepatoprotective, anti-inflammatory and antioxidant properties).
Thyroid Extract, Desiccated
A dehydrated extract of thyroid glands. After the removal of fat and connective tissue, the extract is dried or lyophilized to yield a yellowish to buff-colored amorphous powder containing 0.17-0.23% of iodine.
Arnica
A plant genus of the family ASTERACEAE. The dried flower heads of Arnica montana are used externally as a counterirritant and tincture for sprains and bruises, either as crude extract or in homeopathic dilution (HOMEOPATHY). Arnica contains volatile oils (OILS, VOLATILE), arnicin, arnisterol, FLAVONOIDS; TANNINS; and resin. The common name of Wolf's Bane is similar to the common name for ACONITUM.
Cannabidiol
Compound isolated from Cannabis sativa extract.
Pancreatin
A mammalian pancreatic extract composed of enzymes with protease, amylase and lipase activities. It is used as a digestant in pancreatic malfunction.
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