Track topics on Twitter Track topics that are important to you
Bacteroides are Gram-negative anaerobes indigenous to the intestinal tract of humans and they are important opportunistic pathogens. Mobile genetic elements such as conjugative transposons (CTn's) have contributed to an increase in antibiotic resistance in these organisms. CTn's are self-transmissible elements that belong to the superfamily of Integrating Conjugative Elements (ICE's). CTn341 is 52 kb, encodes tetracycline resistance and its transfer is induced by tetracycline. The mobilization region of CTn341 was shown to be comprised of a three gene operon, mobABC, and the transfer origin, oriT. The three genes code for a nicking accessory protein, relaxase and a VirD4-like coupling protein respectively. The Mob proteins were predicted mediate formation of the relaxosome complex, nick DNA at the oriT, and shuttle the DNA/protein complex to the mating pore apparatus. Mutational studies indicated the three genes are required for maximal transfer of CTn341. Mob gene transcription was induced by tetracycline and this regulation was mediated through the two component regulatory system, RteAB. The oriT region of CTn341 was located within 100 bp of mobA and a putative Bacteroides consensus nicking site was observed within this region. Mutation of the putative nick site resulted in a loss of transfer. This study demonstrated a role of the mobilization region for transfer of Bacteroides CTn's and that tetracycline induction occurs for the tra gene operon(s) as shown previously as well as the mob gene operon as reported here.
Department of Microbiology & Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834.
This article was published in the following journal.
Name: Journal of bacteriology
Conjugation is a dominant mechanism of horizontal gene transfer and substantially contributes to the plasticity and evolvability of prokaryotic genomes. The impact of conjugation on genetic flux exten...
Transposon-mediated "directed" mutations occur at higher frequencies when beneficial than when detrimental and relieve the stress that causes them. The first and best-studied example involves regulati...
Manipulation of biofilm formation in Shewanella is beneficial for applications in industrial and environmental biotechnology. BpfA is an adhesin largely responsible for biofilm formation in many Shewa...
The transfer of genetic material by bacterial conjugation is a process that takes place via complexes formed by specific transfer proteins. In Escherichia coli, these transfer proteins make up a DNA t...
Threonylcarbamoyladenosine is a universally conserved essential modification of tRNA that ensures translational fidelity in cellular milieu. TsaD, TsaB and TsaE are identified as tRNA-A37-threonylcarb...
Gouty diathesis describes uric acid or calcium oxalate nephrolithiasis and low urinary pH (
For the purposes of this study, as a core lab coordinating center, the investigators will be performing P31 MRS core lab analyses; hormone core lab analyses; lipid core lab analyses; gluco...
The goal of the multicenter subproject (SP) 10 of the eMED Alcohol Addiction Consortium - A Systems-Oriented Approach is to study neuroimaging x genetics predictions in an existing sample ...
GENOA, the Genetic Epidemiology Network of Arteriopathy, consists of a network of three field centers and biochemical and genetic core labs to study the common polymorphic genetic variatio...
The purpose of this study is to detect genetic associations for the development of schizophrenia (SZ) and bipolar illness (BP) by comparing Veterans with these diseases to "psychiatrically...
Recombinases that involved in the propagation of DNA TRANSPOSONS. They bind to transposon sequences found at two different sites along the same stretch of DNA and bring them into close proximity. The enzymes then catalyze the double strand cleavage, exchange of double strands and rejoining of DNA helices so that the DNA transposon is formed into a circular PLASMID.
The regulatory elements of an OPERON to which activators or repressors bind thereby effecting the transcription of GENES in the operon.
Bacterial repressor proteins that bind to the LAC OPERON and thereby prevent the synthesis of proteins involved in catabolism of LACTOSE. When lactose levels are high lac repressors undergo an allosteric change that causes their release from the DNA and the resumption of lac operon transcription.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
The discipline studying genetic composition of populations and effects of factors such as GENETIC SELECTION, population size, MUTATION, migration, and GENETIC DRIFT on the frequencies of various GENOTYPES and PHENOTYPES using a variety of GENETIC TECHNIQUES.
MRSA (methicillin-resistant Staphylococcus aureus)
MRSA (methicillin-resistant Staphylococcus aureus) is a family of bacteria with resistance to one or more major antibiotics. There are currently 17 different strains of MRSA. Two particular strains, EMRSA15 and EMRSA16 account for 96% of MRSA blood...
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...