Spectral Analysis of Doxorubicin Accumulation and the Indirect Quantification of its DNA Intercalation.

Summary of "Spectral Analysis of Doxorubicin Accumulation and the Indirect Quantification of its DNA Intercalation."

There is a wide range of techniques utilizing fluorescence of doxorubicin (Dox) commonly used for analysis of intracellular accumulation and destiny of various drug delivery systems containing this anthracycline antibiotic. Unfortunately, results of these studies can be significantly influenced by doxorubicin degradation product, 7,8-dehydro-9,10-desacetyldoxorubicinone (D *) forming spontaneously in aqueous environment, whose fluorescence strongly interfere with that of doxorubicin. Here we define two microscopy techniques enabling to distinguish and separate Dox and D * emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst33342 rather than a direct measurement of doxorubicin fluorescence inside the cells.


Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20, Prague 4, Czech Republic.

Journal Details

This article was published in the following journal.

Name: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst
ISSN: 1873-3441


DeepDyve research library

PubMed Articles [21342 Associated PubMed Articles listed on BioPortfolio]

A Subspace Approach to Spectral Quantification for MR Spectroscopic Imaging.

To provide a new approach for incorporating both spatial and spectral priors into the solution of the spectral quantification problem for magnetic resonance spectroscopic imaging (MRSI).

Multivariate Analysis for Quantification of Plutonium (IV) in Nitric Acid Based on Absorption Spectra.

Development of more effective, reliable, and fast methods for monitoring process streams is a growing opportunity for analytical applications. Many fields can benefit from on-line monitoring, includin...

Fluorescence properties of doxorubicin in PBS buffer and PVA films.

We studied steady-state and time-resolved fluorescence properties of an anticancer drug Doxorubicin in a saline buffer and poly-vinyl alcohol (PVA) film. Absorption of Doxorubicin, located at blue-gre...

ATP-dependent activity and mitochondrial localization of drug efflux pumps in doxorubicin-resistant breast cancer cells.

We hypothesized that, among the mechanisms of drug-resistance acquired by doxorubicin (DOX)-resistant breast cancer cells to maintain cell survival, ATP-dependent drug efflux pumps could be expressed ...

UV Spectrometric Indirect Analysis of Brominated MWCNTs with UV Active Thiols and an Alkene-Reaction Kinetics, Quantification and Differentiation of Adsorbed Bromine and Oxygen.

Indirect UV-absorption spectrometry was shown to be a valuable tool for chemical characterization of functionalized carbon nanotubes (CNTs). It complements data from X-ray photoelectron spectroscopy (...

Clinical Trials [3123 Associated Clinical Trials listed on BioPortfolio]

Adjuvant Erlotinib Intercalating Chemotherapy or Adjuvant Chemotherapy Alone in NSCLC With Common EGFR Mutation

This study will be performed as a local multicenter, randomized, phase III clinical study. It will compare the adjuvant chemotherapy in Stage IB-IIIA NSCLC with common EGFR mutation (Exon ...

Quantification of Autonomic Nervous Activity During Hemodialysis

This study assesses autonomic nervous system function by power spectral analysis of RR interval dynamics in ultrafiltration subjects without blood pressure variation.

Spectral Diagnosis of Cutaneous Malignancy

The goal of this clinical research study is to evaluate the use of an imaging technology called spectral diagnosis. Researchers want to find out if a special spectral-diagnosis probe can ...

Cardiac Autonomic Control in Children of HIV Positive Mothers

To establish the incidence, clinical spectrum, and natural history of cardiac dysautonomia as defined by heart rate spectral analysis in both HIV infected and noninfected children and to e...

Relaxation and Heart Rate Variability

Primary: - To characterize the physiologic changes of the autonomic nervous system, demonstrated by heart rate variability (HRV) high frequency (HF) spectral analysis, before and ...

Medical and Biotech [MESH] Definitions

Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN.

Accumulation of BILIRUBIN, a breakdown product of HEME PROTEINS, in the BLOOD during the first weeks of life. This may lead to NEONATAL JAUNDICE. The excess bilirubin may exist in the unconjugated (indirect) or the conjugated (direct) form. The condition may be self-limiting (PHYSIOLOGICAL NEONATAL JAUNDICE) or pathological with toxic levels of bilirubin.

An anthracycline which is the 4'-epi-isomer of doxorubicin. The compound exerts its antitumor effects by interference with the synthesis and function of DNA.

A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Method to determine the occurrence of OVULATION by direct or indirect means. Indirect methods examine the effects of PROGESTERONE on cervical mucus (CERVIX MUCUS), or basal body temperature. Direct ovulation detection, generally used in fertility treatment, involves analyses of circulating hormones in blood and ULTRASONOGRAPHY.

Quick Search

DeepDyve research library

Relevant Topics

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Drug Discovery
Clinical Approvals Clinical Trials Drug Approvals Drug Delivery Drug Discovery Generics Drugs Prescription Drugs In the fields of medicine, biotechnology and pharmacology, drug discovery is the process by which drugs are dis...

Searches Linking to this Article