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Spectral Analysis of Doxorubicin Accumulation and the Indirect Quantification of its DNA Intercalation.

Summary of "Spectral Analysis of Doxorubicin Accumulation and the Indirect Quantification of its DNA Intercalation."

There is a wide range of techniques utilizing fluorescence of doxorubicin (Dox) commonly used for analysis of intracellular accumulation and destiny of various drug delivery systems containing this anthracycline antibiotic. Unfortunately, results of these studies can be significantly influenced by doxorubicin degradation product, 7,8-dehydro-9,10-desacetyldoxorubicinone (D *) forming spontaneously in aqueous environment, whose fluorescence strongly interfere with that of doxorubicin. Here we define two microscopy techniques enabling to distinguish and separate Dox and D * emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst33342 rather than a direct measurement of doxorubicin fluorescence inside the cells.

Affiliation

Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20, Prague 4, Czech Republic.

Journal Details

This article was published in the following journal.

Name: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst
ISSN: 1873-3441
Pages:

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Accumulation of BILIRUBIN, a breakdown product of HEME PROTEINS, in the BLOOD during the first weeks of life. This may lead to NEONATAL JAUNDICE. The excess bilirubin may exist in the unconjugated (indirect) or the conjugated (direct) form. The condition may be self-limiting (PHYSIOLOGICAL NEONATAL JAUNDICE) or pathological with toxic levels of bilirubin.

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