Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice.
Summary of "Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice."
ABSTRACT:
OBJECTIVE:
The purpose of the study was to explore the anti-tumor effect of ultrasound-targeted microbubble destruction mediated herpes simplex virus--thymidine kinase (HSV-TK) suicide gene system on mice hepatoma.
METHODS:
Forty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were estabilished:(1)PBS;(2) HSV-TK (3) HSV-TK+ultrasound(HSV-TK + US);(4)HSV-TK+ultrasound+microbubbles(HSV-TK+US+MB).The TK protein expression in liver cancer was detected by western-blot. Applying TUNEL staining detected tumor cell apoptosis.At last, the inhibition rates and survival time of the animals were compared among all groups.
RESULTS:
The TK protein expression of HSV-TK+MB+US group in tumor-bearing mice tissues were significantly higher than those in other groups. The tumor inhibitory effect of ultrasound-targeted microbubble destruction mediated HSV-TK on mice transplantable tumor was significantly higher than those in other groups (p <0.05), and can significantly improve the survival time of tumor-bearing mice.
CONCLUSION:
Ultrasound-targeted microbubble destruction can effectively transfect HSV-TK gene into target tissues and play a significant inhibition effect on tumors, which provides a new strategy for gene therapy in liver cancer.
Affiliation
Journal Details
This article was published in the following journal.
Name: Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Pages: 170
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21176239
- DOI: http://dx.doi.org/10.1186/1756-9966-29-170
Medical and Biotech [MESH] Definitions
Herpes Simplex Virus Protein Vmw65
Trans-acting protein that combines with host factors to induce immediate early gene transcription in herpes simplex virus.
Host Cell Factor C1
A cellular transcriptional coactivator that was originally identified by its requirement for the stable assembly IMMEDIATE-EARLY PROTEINS of the HERPES SIMPLEX VIRUS. It is a nuclear protein that is a transcriptional coactivator for a number of transcription factors including VP16 PROTEIN; GA-BINDING PROTEIN; EARLY GROWTH RESPONSE PROTEIN 2; and E2F4 TRANSCRIPTION FACTOR. It also interacts with and stabilizes HERPES SIMPLEX VIRUS PROTEIN VMW65 and helps regulate GENETIC TRANSCRIPTION of IMMEDIATE-EARLY GENES in HERPES SIMPLEX VIRUS.
Herpes Genitalis
Infection of the genitals (GENITALIA) with HERPES SIMPLEX VIRUS in either the males or the females.
Herpes Simplex
A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)
Herpes Labialis
Herpes simplex, caused by type 1 virus, primarily spread by oral secretions and usually occurring as a concomitant of fever. It may also develop in the absence of fever or prior illness. It commonly involves the facial region, especially the lips and the nares. (Dorland, 27th ed.)
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