Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice.
Summary of "Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice."
The purpose of the study was to explore the anti-tumor effect of ultrasound-targeted microbubble destruction mediated herpes simplex virus--thymidine kinase (HSV-TK) suicide gene system on mice hepatoma.
Forty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were estabilished:(1)PBS;(2) HSV-TK (3) HSV-TK+ultrasound(HSV-TK + US);(4)HSV-TK+ultrasound+microbubbles(HSV-TK+US+MB).The TK protein expression in liver cancer was detected by western-blot. Applying TUNEL staining detected tumor cell apoptosis.At last, the inhibition rates and survival time of the animals were compared among all groups.
The TK protein expression of HSV-TK+MB+US group in tumor-bearing mice tissues were significantly higher than those in other groups. The tumor inhibitory effect of ultrasound-targeted microbubble destruction mediated HSV-TK on mice transplantable tumor was significantly higher than those in other groups (p <0.05), and can significantly improve the survival time of tumor-bearing mice.
Ultrasound-targeted microbubble destruction can effectively transfect HSV-TK gene into target tissues and play a significant inhibition effect on tumors, which provides a new strategy for gene therapy in liver cancer.
This article was published in the following journal.
Name: Journal of experimental & clinical cancer research : CR
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21176239
- DOI: http://dx.doi.org/10.1186/1756-9966-29-170
Medical and Biotech [MESH] Definitions
Herpes Simplex Virus Protein Vmw65
Trans-acting protein that combines with host factors to induce immediate early gene transcription in herpes simplex virus.
Host Cell Factor C1
A cellular transcriptional coactivator that was originally identified by its requirement for the stable assembly IMMEDIATE-EARLY PROTEINS of the HERPES SIMPLEX VIRUS. It is a nuclear protein that is a transcriptional coactivator for a number of transcription factors including VP16 PROTEIN; GA-BINDING PROTEIN; EARLY GROWTH RESPONSE PROTEIN 2; and E2F4 TRANSCRIPTION FACTOR. It also interacts with and stabilizes HERPES SIMPLEX VIRUS PROTEIN VMW65 and helps regulate GENETIC TRANSCRIPTION of IMMEDIATE-EARLY GENES in HERPES SIMPLEX VIRUS.
Infection of the genitals (GENITALIA) with HERPES SIMPLEX VIRUS in either the males or the females.
A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)
Herpes simplex, caused by type 1 virus, primarily spread by oral secretions and usually occurring as a concomitant of fever. It may also develop in the absence of fever or prior illness. It commonly involves the facial region, especially the lips and the nares. (Dorland, 27th ed.)
Novel "soft" microbubbles have been fabricated to show outstanding ultrasound imaging capability, and triggered CuS nanoparticles delivery through ultrasound-targeted microbubble destruction for effic...
Since herpes simplex virus type 2 (HSV-2) antibody tests have become commercially available, an increasing number of persons have learned that they have genital herpes through serologic testing. The c...
A 16-year-old girl with congenital T-cell immunodeficiency presented with a 2-month history of an enlarging lower eyelid mass. After complete excision of the mass, the lesion was noted to recur. Histo...
to determine the impact of oral tenofovir as part of combination antiretroviral therapy on asymptomatic herpes simplex virus (HSV) shedding.
We describe a rare case of a 79-year-old woman who developed herpes simplex virus pneumonia after mitral valve replacement. The patient showed persistent hypoxemia with bilateral glass-like shadows on...
RATIONALE: Acyclovir may be effective in preventing herpes simplex virus infection in patients with neutropenia. PURPOSE: This randomized phase III trial is studying the side effects of a...
To evaluate the safety and tolerance of topically applied SP-303T in AIDS patients. To observe the effect of this drug on herpes simplex virus lesions in patients who have failed to heal i...
A Phase I/II Study of the Safety and Efficacy of Topical 1-(S)-(3-Hydroxy-2-Phosphonylmethoxypropyl)Cytosine Dihydrate (Cidofovir; HPMPC) in the Treatment of Refractory Mucocutaneous Herpes Simplex Disease in Patients With AIDS
To evaluate the safety and tolerance of topical cidofovir (HPMPC) therapy for refractory mucocutaneous herpes simplex virus disease in AIDS patients. To determine whether topical HPMPC the...
The purpose of this study is to see if valacyclovir (Valtrex) is a safe and effective treatment for ano-genital HSV infections (herpes simplex virus infections of the anus and external gen...
Genital herpes (GH) is a commonly occurring sexually transmitted disease caused by herpes simplex virus (HSV). There are two types of HSV, type 1 (HSV-1) and type 2 (HSV-2); both can cause...