A catechol biosensor based on a gold nanoparticles encapsulated-dendrimer.
Summary of "A catechol biosensor based on a gold nanoparticles encapsulated-dendrimer."
Tyrosinase has been immobilized on a Au nanoparticles encapsulated-dendrimer bonded conducting polymer on a glassy carbon electrode for the estimation of catechol. The modified electrode was characterized by cyclic voltammetry and AFM techniques. The principle of catechol estimation was based on the reduction of biocatalytically liberated quinone species at +0.2 V versus Ag/AgCl (3 M KCl), with good stability, sensitivity, and featuring a low detection limit (about 0.002 μM) and wide linear range (0.005 μM-120 μM). The electrochemical redox peak of catechol on the GCE/PolyPATT/Den(AuNPs)/tyrosinase was also investigated. A response time of 7 s, reusability up to 5 cycles and a shelf life of more than 2 months under refrigerated conditions were reported. Various parameters influencing biosensor performance have been optimized including pH, temperature, and applied potential. The utility and application of this nanobiosensor was tested in a real water samples.
Affiliation
Department of Chemistry and Center for Innovative Biophysio Sensor Technology, Pusan National University, Busan, 609-735, S. Korea.
Journal Details
This article was published in the following journal.
Name: The Analyst
ISSN: 1364-5528
Pages: 1216-21
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21240422
- DOI: http://dx.doi.org/10.1039/c0an00601g
Medical and Biotech [MESH] Definitions
Metal Nanoparticles
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
Nanoparticles
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Metal Ceramic Alloys
The fusion of ceramics (porcelain) to an alloy of two or more metals for use in restorative and prosthodontic dentistry. Examples of metal alloys employed include cobalt-chromium, gold-palladium, gold-platinum-palladium, and nickel-based alloys.
Catechol O-methyltransferase
Enzyme that catalyzes the movement of a methyl group from S-adenosylmethionone to a catechol or a catecholamine. EC 2.1.1.6.
Gold Radioisotopes
Unstable isotopes of gold that decay or disintegrate emitting radiation. Au 185-196, 198-201, and 203 are radioactive gold isotopes.
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