Advertisement

Topics

Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe.

Summary of "Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe."

We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

Affiliation

Department of Chemistry, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL, 32816, USA.

Journal Details

This article was published in the following journal.

Name: Journal of fluorescence
ISSN: 1573-4994
Pages:

Links

DeepDyve research library

PubMed Articles [4772 Associated PubMed Articles listed on BioPortfolio]

Single-molecule analysis of fluorescent carbon dots towards localization-based super-resolution microscopy.

The advancement of high-resolution bioimaging has always been dependent on the discovery of bright and easily available fluorescent probes. Fluorescent carbon nanodots, an interesting class of relativ...

Development of a two-photon fluorescent turn-on probe with far-red emission for thiophenols and its bioimaging application in living tissues.

Thiophenol is a highly toxic compound which is essential in the field of organic synthesis and drug design. However, the accumulation of thiophenols in the environment may cause serious health problem...

A novel self-quenching system based on bis-naphthalimide: a dual two-photon-channel GSH fluorescent probe.

The single fluorescent signal output often does not satisfy the requirement of detection accuracy for bioactive compounds in biological system. It is of great interest to develop a dual-channel turn-o...

A naphthalene-based two-photon fluorescent probe for selective and sensitive detection of endogenous hypochlorous acid.

An efficient naphthalene-based two-photon fluorescent probe for endogenous HClO has been reported in the present study, which consists of a 6-(2-benzothiazolyl)-2-naphthalenol fluorophore connected wi...

A hemicyanine-based colorimetric and ratiometric fluorescent probe for selective detection of cysteine and bioimaging in living cell.

A new ratiometric fluorescent probe based on a hemicyanine dye was synthesized. Investigation on the response behavior towards amino acids showed that the probe had a capability of rapidly and selecti...

Clinical Trials [517 Associated Clinical Trials listed on BioPortfolio]

Fluorescence Guided Surgery in Breast Cancer

A need for further investigation for fluorescence image-guided surgery in breast conserving surgery (BCS) has arisen following the results obtained from a phase I feasibility breast cancer...

Visualization of Rectal Cancer During Endoscopy, Using a Fluorescent Tracer

To improve rectal cancer management, there is a need for better visualization of drug targets in rectal cancer to identify patients who might benefit from specific targeted treatments. Mol...

Ultra-Violet Fluorescence Bronchial Cancer Location

Diagnose lung cancer at an early stage is crucial for effective treatment. At these early stages, cancer is usually invisible in ambient white light when the endoscopic analysis of the bro...

Paclitaxel-Loaded Polymeric Micelle and Carboplatin as First-Line Therapy in Treating Patients With Advanced Ovarian Cancer

RATIONALE: Drugs used in chemotherapy, such as paclitaxel-loaded polymeric micelle and carboplatin, work in different ways to stop the growth of tumor cells, either by killing the cells or...

A Clinical Trial of Paclitaxel Loaded Polymeric Micelle in Patients With Taxane-Pretreated Recurrent Breast Cancer

The purpose of this study is to evaluate the response rate in patients with taxane-pretreated recurrent breast cancer receiving paclitaxel loaded polymeric micelle (Genexol-PM).

Medical and Biotech [MESH] Definitions

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.

Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.

Quick Search
Advertisement
 


DeepDyve research library

Searches Linking to this Article