Detection of Staphylococcus hyicus exfoliative toxin genes by dot blot hybridization and multiplex polymerase chain reaction.
Summary of "Detection of Staphylococcus hyicus exfoliative toxin genes by dot blot hybridization and multiplex polymerase chain reaction."
We designed a novel DNA probe and novel PCR primer sets to detect the genes coding for S. hyicus exfoliative toxin (ET). In dot blot hybridization, the novel DNA probe hybridized with chromosomal DNA of ExhA-, ExhB-, ExhC-, ExhD-, and SHETA-producing strains. This probe also hybridized with plasmid DNA of a SHETB-producing strain. In Southern blot hybridization, the probe hybridized with a 1.5-kb HindIII fragment of chromosomal DNA from a SHETA-producing strain. We cloned the above fragment into E. coli and determined the nucleotide sequence of the SHETA gene, which proved to be almost the same (99.6% homology) as that of the ExhB gene. So it was thought that SHETA is a subtype of ExhB. In multiplex PCR using five primer sets, each gene gave a band distinguishable from the others. This multiplex PCR system had high specificity among the well-known S. hyicus ET genes. Of the 69 known ET-producing S. hyicus strains, 38, 19, 9, 2 and 1 strains have exhB, exhD exhA, shetb and exhC genes, respectively.
Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, School of Veterinary Medicine, Kitasato University, 35-1, Higashi 23 Ban-cho, Towada, 034-8628 Aomori, Japan.
This article was published in the following journal.
Name: Microbiology and immunology
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21244467
- DOI: http://dx.doi.org/10.1111/j.1348-0421.2011.00308.x
A simple experiment was carried out to examlne the effect of varlous physicochemical conditions on toxigenicity and toxin genes of Vibrio cholerae 01 lsolated from an artificial aquatic environment. A...
Staphylococcus aureus causes infections both in community and hospital settings, nasal carriage is the important source of these infections. A total of 103 carrier isolates of S. aureus from 352 asymp...
Some studies have shown that the NetB toxin may be an important virulence factor of Clostridium perfringens associated necrotic enteritis in poultry. Additionally, research has shown that strains of C...
Development and evaluation of hexaplex PCR for rapid detection of methicillin, cadmium/ zinc and antiseptic resistant staphylococci, with simultaneous identification of PVL-positive and- negative Staphylococcus aureus and coagulase negative staphylococci.
We developed a multiplex PCR to detect the presence of methicillin (mecA), cadmium/zinc (czrC) and antiseptic resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positiv...
The objectives of this study were to determine the occurrence and diversity of Staphylococcus spp. in milk from healthy cows and cows with subclinical mastitis in Brazil and to examine the profile of...
To establish the clinical test performance characteristics of sensitivity, specificity and predictive values for direct detection of nasal Staphylococcus aureus colonization against clinic...
The purpose of this study is to determine if rapid early detection of the bacteria causing sepsis in burn patients improves patient outcomes.
Botulism is a severe form of food poisoning caused by bacteria. This bacteria produces several toxins one of which is botulinum toxin A. This toxin causes the symptoms of food poisoning....
1.To assess whether the Elution swab (ESwab) is superior to Amies transport swabs for the detection of Methicillin Resistant Staphylococcus aureus (MRSA) and Vancomycin Resistant Enterococ...
RATIONALE: Studying the genes expressed in samples of tissue from patients with abnormal cells may help doctors identify biomarkers related to cancer. PURPOSE: This clinical trial is iden...
Medical and Biotech [MESH] Definitions
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
A species of gram-positive bacteria in the family STAPHYLOCOCCACEAE. It is an important opportunistic pathogen in swine.
A species of STAPHYLOCOCCUS similar to STAPHYLOCOCCUS HAEMOLYTICUS, but containing different esterases. The subspecies Staphylococcus hominis novobiosepticus is highly virulent and novobiocin resistant.
Protein exotoxins from Staphylococcus aureus, phage type II, which cause epidermal necrolysis. They are proteins with a molecular weight of 26,000 to 32,000. They cause a condition variously called scaled skin, Lyell or Ritter syndrome, epidermal exfoliative disease, toxic epidermal necrolysis, etc.