Advertisement

Topics

Procaine Effect on Human Erythrocyte Membrane Explored by Atomic Force Microscopy.

Summary of "Procaine Effect on Human Erythrocyte Membrane Explored by Atomic Force Microscopy."

The procaine effect on human erythrocytes was investigated by atomic force microscopy (AFM) at three procaine concentrations, about 5 x 10(-7) M, 5 x 10(-5) M and 5 x 10(-4) M. The changes in surface morphology of erythrocyte membrane bring direct evidence on the procaine effect on the cell membrane at micro- and nanometer scale. AFM images of the control erythrocytes (without procaine) showed a well defined concave (donut) shape of cells. The structure of control erythrocytes membrane is featured by closely packed nanometer size intra-membranous particles. After the incubation of the fresh blood with increasing procaine concentrations, a progressive increase in both concave depth and surface roughness of erythrocyte membrane was observed. The particles (granules) of the membrane surface increased progressively with increasing procaine concentrations. The changes in the surface morphology of erythrocyte membrane can be associated with the enlargement of surface granules, due to the aggregation of membranous particles within the cell surface, and the domain structure formation induced by procaine. A large number of moderate elevations from 25 nm to almost 40 nm in lateral size were found to be rather uniformly distributed on the surface of whole erythrocytes at low and medium procaine concentrations, respectively. At the highest procaine concentration, the granules of about 80 nm to almost 90 nm lateral size were found to form rows rather well separated. These data are in substantial agreement with the published results obtained on membrane models in the presence of procaine.

Affiliation

Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, 400028 Cluj-Napoca, Romania. ossihor@yahoo.com.

Journal Details

This article was published in the following journal.

Name: Combinatorial chemistry & high throughput screening
ISSN: 1875-5402
Pages:

Links

DeepDyve research library

PubMed Articles [30399 Associated PubMed Articles listed on BioPortfolio]

The role of CD4 on mechanical properties of live cell membrane.

Although much progress has been made in the characterization and identification of CD4 functions, its role in mechanical properties of cell membrane remains largely unknown. Here an atomic force micro...

Evaluating the morphology of erythrocyte population: An approach based on atomic force microscopy and flow cytometry.

Erythrocyte morphology is gaining importance as a powerful pathological index in identifying the severity of any blood related disease. However, the existing technique of quantitative microscopy is hi...

Atomic Force Microscopic Analysis of the Effect of Lipid Composition on Liposome Membrane Rigidity.

Mechanical rigidity of the liposome membrane is often defined by the membrane bending modulus and is one of the determinants of liposome stability, but the quantitative experimental data are still lim...

The modulation of erythrocyte Na(+)/K(+)-ATPase activity by curcumin.

Curcumin, an active biphenolic molecule present in turmeric (Curcuma longa), has been reported to elicit plethora of health protective effects. The present study was carried out in vitro, in vivo and ...

Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes.

ATP release from erythrocyte plays a key role in hypoxia-induced elevation of blood flow in systematic circulation. We have previously shown that hemolysis contributes to erythrocyte ATP release trigg...

Clinical Trials [3844 Associated Clinical Trials listed on BioPortfolio]

Simplified Antibiotic Therapy for Sepsis in Young Infants

This trial evaluates primary care clinic-based simplified antibiotic therapy options for young infants, 0-59 days old in high neonatal mortality settings in peri-urban Karachi where hospit...

Miami Membrane for Potency (MMEP) Trial

The use of dehydrated human amnionic membrane allograft will result in an absolute 20% increase (from 40-60%) in the proportion of men considered potent (Sexual History Inventory for Men (...

Utilization of Amniotic Membrane Extract Eye Drop (AMEED) on Human Corneal Healing

For severe ocular surface diseases, such as chemical and thermal injuries, Stevens-Johnson syndrome (SJS), Band Keratopathy, Corneal Dystrophies, Refractive (PRK-LASEK), corneal surgery an...

Effect of Cyclic Tensional Force on Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

The purpose of this study is to observe cyclic tensional force on the osteogenic differentiation of human periodontal ligament stem cells.

Comparison of Two Different Alveolar Ridge Preservation Techniques

The purpose of this study is to compare two barrier membrane products, Cytoplast (dPTFE) and BioXclude (human amnion chorion allograft), for their relative efficacies in dental socket pres...

Medical and Biotech [MESH] Definitions

The formation of clumps of RED BLOOD CELLS under low or non-flow conditions, resulting from the attraction forces between the red blood cells. The cells adhere to each other in rouleaux aggregates. Slight mechanical force, such as occurs in the circulation, is enough to disperse these aggregates. Stronger or weaker than normal aggregation may result from a variety of effects in the ERYTHROCYTE MEMBRANE or in BLOOD PLASMA. The degree of aggregation is affected by ERYTHROCYTE DEFORMABILITY, erythrocyte membrane sialylation, masking of negative surface charge by plasma proteins, etc. BLOOD VISCOSITY and the ERYTHROCYTE SEDIMENTATION RATE are affected by the amount of erythrocyte aggregation and are parameters used to measure the aggregation.

A ubiquitous membrane transport protein found in the plasma membrane of diverse cell types and tissues, and in nuclear, mitochondrial, and Golgi membranes. It is the major integral transmembrane protein of the erythrocyte plasma membrane, comprising 25% of the total membrane protein. It exists as a dimer and performs the important function of allowing the efficient transport of bicarbonate across erythrocyte cell membranes in exchange for chloride ion.

A family of membrane-associated proteins responsible for the attachment of the cytoskeleton. Erythrocyte-related isoforms of ankyrin attach the SPECTRIN cytoskeleton to a transmembrane protein (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE) in the erythrocyte plasma membrane. Brain-related isoforms of ankyrin also exist.

A high molecular weight (220-250 kDa) water-soluble protein which can be extracted from erythrocyte ghosts in low ionic strength buffers. The protein contains no lipids or carbohydrates, is the predominant species of peripheral erythrocyte membrane proteins, and exists as a fibrous coating on the inner, cytoplasmic surface of the membrane.

Semisynthetic antibiotic prepared by combining penicillin G with PROCAINE.

Quick Search
Advertisement
 
DeepDyve research library

Relevant Topics

Blood
Blood is a specialized bodily fluid that delivers necessary substances to the body's cells (in animals) – such as nutrients and oxygen – and transports waste products away from those same cells.  In vertebrates, it is composed of blo...

Collaborations in biotechnology
Commercial and academic collaborations are used throughout the biotechnology and pharmaceutical sector to enhance research and product development. Collaborations can take the form of research and evaluation agreements, licensing, partnerships etc. ...


Searches Linking to this Article