Effect of Nystatin on Guinea Pigs' Inner Ear.
Summary of "Effect of Nystatin on Guinea Pigs' Inner Ear."
Nystatin is a topical antifungal agent. Its wide spectrum of action and low cost make it a treatment of choice. Though it is safe for external ear use, no study has proven its safety in cases of tympanic membrane perforation (TMP). We aim to test the safety of Nystatin when applied directly to the middle ear of a Guinea Pig model. We performed an experimental study with 18 Hartley Guinea Pigs that were divided into two groups. All tympanic membranes were perforated at the beginning of the study. Exposing one group to Nystatin and the other to the ototoxic Neomycin, we compared results of auditory brainstem response testing at three intervals. Each animals' contralateral ear was used as a negative control. At the end, we performed a histological analysis of the animals' cochleae using a scanning electron microscope (SEM). Average hearing loss in the Nystatin group was 13.0 dB which was similar to the results obtained in the negative control group (13.1 dB). Average hearing loss in the Neomycin group was 39.3 dB, which represents a statistically significant difference (P < 0.001). SEM evaluation revealed intact cochlear hair cell architecture in the Nystatin and normal saline groups, whereas significant hair cell losses were noted in the Neomycin group was noted. Nystatin does not cause hearing impairment or cochlear hair cell damage when exposed directly to the middle ear of a Guinea Pig model. It is therefore a safe treatment option for otomycosis with presence of TMP.
Affiliation
Department of Otolaryngology Head and Neck Surgery, Sainte-Justine University Hospital Center (CHU SJ), Montreal University, 3175, Côte Sainte-Catherine, Montreal, QC, H3T 1C5, Canada.
Journal Details
This article was published in the following journal.
Name: Neurotoxicity research
ISSN: 1476-3524
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21384237
- DOI: http://dx.doi.org/10.1007/s12640-011-9242-2
Medical and Biotech [MESH] Definitions
Guinea Pigs
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Nystatin
Macrolide antifungal antibiotic complex produced by Streptomyces noursei, S. aureus, and other Streptomyces species. The biologically active components of the complex are nystatin A1, A2, and A3.
New Guinea
Originally an island of the Malay Archipelago, the second largest island in the world. It divided, West New Guinea becoming part of Indonesia and East New Guinea becoming Papua New Guinea.
Papua New Guinea
A country consisting of the eastern half of the island of New Guinea and adjacent islands, including New Britain, New Ireland, the Admiralty Islands, and New Hanover in the Bismarck Archipelago; Bougainville and Buka in the northern Solomon Islands; the D'Entrecasteaux and Trobriand Islands; Woodlark (Murua) Island; and the Louisiade Archipelago. It became independent on September 16, 1975. Formerly, the southern part was the Australian Territory of Papua, and the northern part was the UN Trust Territory of New Guinea, administered by Australia. They were administratively merged in 1949 and named Papua and New Guinea, and renamed Papua New Guinea in 1971.
Zeta-crystallins
A group of crystallins that have been found in the lens (LENS, CRYSTALLINE) of certain species of VERTEBRATES including GUINEA PIGS; CAMELS; and LLAMAS. They are inactivated forms of NAD(P)H DEHYDROGENASE (QUINONE).
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