Profiling LC-DAD-ESI-TOF MS Method for the Determination of Phenolic Metabolites from Avocado ( Persea americana ).

Summary

A powerful HPLC-DAD-ESI-TOF MS method was established for the efficient identification of the chemical constituents in the methanolic extracts of avocado ( Persea americana ). Separation and detection conditions were optimized by using a standard mix containing 39 compounds belonging to phenolic acids and different categories of flavonoids, analytes that could be potentially present in the avocado extracts. Optimum LC separation was achieved on a Zorbax Eclipse Plus C18 analytical column (4.6 Ã� 150 mm, 1.8 Î¼m particle size) by gradient elution with water + acetic acid (0.5%) and acetonitrile as mobile phases, at a flow rate of 1.6 mL/min. The detection was carried out by ultraviolet-visible absorption and ESI-TOF MS. The developed method was applied to the study of 3 different varieties of avocado, and 17 compounds were unequivocally identified with standards. Moreover, around 25 analytes were tentatively identified by taking into account the accuracy and isotopic information provided by TOF MS.

Affiliation

Department of Analytical Chemistry, Faculty of Sciences, University of Granada , c/Fuentenueva s/n, E-18071 Granada, Spain.

Journal Details

Name: Journal of agricultural and food chemistry
ISSN: 1520-5118
Pages: 2255-2267

MESH Definitions

Persea

A plant genus in the LAURACEAE family. The tree, Persea americana Mill., is known for the Avocado fruit, the food of commerce.

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