miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFKB1.
Summary of "miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFKB1."
The activation of nuclear factor-kappa B1 (NFKB1) in cancer cells may confer resistance to ionizing radiation (IR). To enhance the therapeutic efficiency of IR in lung cancer, we screened for microRNAs (miRNAs) that suppress NFKB1 and observed their effects on radiosensitivity in a human lung cancer cell line. From time series data of miRNA expression in g-irradiated H1299 human lung cancer cells, we found that the expression of miR-9 was inversely correlated with that of NFKB1. Overexpression of miR-9 down-regulated the level of NFKB1 in H1299 cells, and the surviving fraction of γ-irradiated cells was decreased. Interestingly, let-7g also suppressed the expression of NFKB1, although there was no canonical target site for let-7g in the NFKB1 3' untranslated region. From these results, we conclude that the expression of miR-9 and let-7g could enhance the efficiency of radiotherapy for lung cancer treatment through the inhibition of NFKB1.
This article was published in the following journal.
Name: Experimental & molecular medicine
Medical and Biotech [MESH] Definitions
A genus of gram-positive aerobic cocci found in the soil, that is highly resistant to radiation, especially ionizing radiation (RADIATION, IONIZING). Deinococcus radiodurans is the type species.
Radiation Injuries, Experimental
Experimentally produced harmful effects of ionizing or non-ionizing RADIATION in CHORDATA animals.
Congenital changes in the morphology of organs produced by exposure to ionizing or non-ionizing radiation.
Harmful effects of non-experimental exposure to ionizing or non-ionizing radiation in VERTEBRATES.
Inflammation of the lung due to harmful effects of ionizing or non-ionizing radiation.
Osteosarcoma is the common primary bone malignancy in children and young adults in Eastern countries. Resistance to ionizing radiation (IR) or drugs is an underlying mechanism contributing to the fail...
AKT phosphorylates components of the intrinsic cell survival machinery and promotes survival to various stimuli. In the present study, we identified CDC-like kinase 2 (CLK2) as a new substrate of AKT...
We evaluated the combined effect of As(2)O(3) and ionizing radiation on cultured renal carcinoma cells. The cells receiving both As(2)O(3) and radiotherapy had more DNA damage, more apoptosis, more re...
Ionizing radiation causes DNA damage and consequent apoptosis, mainly due to the production of hydroxyl radicals that follows radiolytic splitting of water. However, superoxide and hydrogen peroxide a...
Skin changes caused by ionizing radiation have been scientifically documented since 1902. Ionizing radiation is a widely accepted form of treatment for various types of cancer. Despite the technologic...
Doctors will take some tissue from the tissue removed during surgery in order to study how the blood vessels of the tumor respond to radiation therapy. The tissue obtained will be used to...
Although ionizing radiations have been proposed for the prevention of intimal hyperplasia in coronary and peripheral arteries, information is lacking on how irradiation may prevent neointi...
Ionizing radiation produces cancer cell death by creating high levels of reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, in irradiated cells. Cancer cells are pref...
This randomized study is looking at the benefits of using docetaxel (chemotherapy) added to one of the standard treatments (radiation and hormones) for men with high-risk prostate cancer.
RATIONALE: Androgens can cause the growth of prostate cancer cells. Antihormone therapy, such as flutamide, bicalutamide, leuprolide, buserelin, and goserelin, may lessen the amount of and...