miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFKB1.
Summary of "miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFKB1."
The activation of nuclear factor-kappa B1 (NFKB1) in cancer cells may confer resistance to ionizing radiation (IR). To enhance the therapeutic efficiency of IR in lung cancer, we screened for microRNAs (miRNAs) that suppress NFKB1 and observed their effects on radiosensitivity in a human lung cancer cell line. From time series data of miRNA expression in g-irradiated H1299 human lung cancer cells, we found that the expression of miR-9 was inversely correlated with that of NFKB1. Overexpression of miR-9 down-regulated the level of NFKB1 in H1299 cells, and the surviving fraction of γ-irradiated cells was decreased. Interestingly, let-7g also suppressed the expression of NFKB1, although there was no canonical target site for let-7g in the NFKB1 3' untranslated region. From these results, we conclude that the expression of miR-9 and let-7g could enhance the efficiency of radiotherapy for lung cancer treatment through the inhibition of NFKB1.
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Journal Details
This article was published in the following journal.
Name: Experimental & molecular medicine
ISSN: 1226-3613
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21464588
- DOI: http://dx.doi.org/
Medical and Biotech [MESH] Definitions
Deinococcus
A genus of gram-positive aerobic cocci found in the soil, that is highly resistant to radiation, especially ionizing radiation (RADIATION, IONIZING). Deinococcus radiodurans is the type species.
Radiation Injuries, Experimental
Experimentally produced harmful effects of ionizing or non-ionizing RADIATION in CHORDATA animals.
Abnormalities, Radiation-induced
Congenital changes in the morphology of organs produced by exposure to ionizing or non-ionizing radiation.
Radiation Injuries
Harmful effects of non-experimental exposure to ionizing or non-ionizing radiation in VERTEBRATES.
Radiation Pneumonitis
Inflammation of the lung due to harmful effects of ionizing or non-ionizing radiation.
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