SV40 host-substituted variants: a new look at the monkey DNA inserts and recombinant junctions.
Summary of "SV40 host-substituted variants: a new look at the monkey DNA inserts and recombinant junctions."
The available monkey genomic data banks were examined in order to determine the chromosomal locations of the host DNA inserts in 8 host-substituted SV40 variant DNAs. Five of the 8 variants contained more than one linked monkey DNA insert per tandem repeat unit and in all cases but one, the 19 monkey DNA inserts in the 8 variants mapped to different locations in the monkey genome. The 50 parental DNAs (32 monkey and 18 SV40 DNA segments) which spanned the crossover and flanking regions that participated in monkey/monkey and monkey/SV40 recombinations were characterized by substantial levels of microhomology of up to 8 nucleotides in length; the parental DNAs also exhibited direct and inverted repeats at or adjacent to the crossover sequences. We discuss how the host-substituted SV40 variants arose and the nature of the recombination mechanisms involved.
Affiliation
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA; Carnegie Institution for Science, 1530 P Street, NW, Washington, DC 20005, USA.
Journal Details
This article was published in the following journal.
Name: Virology
ISSN: 1096-0341
Pages: 325-32
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21316069
- DOI: http://dx.doi.org/10.1016/j.virol.2011.01.020
Medical and Biotech [MESH] Definitions
Cos Cells
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Colobinae
A subfamily of the Old World monkeys, CERCOPITHECIDAE, that inhabits the forests of Africa and Asia. The genera COLOBUS (Procolobus; colobus), Nasalis (proboscis monkey), Presbytis (Semnopithecus; leaf monkey), Pygathrix (Rhinopithecus; snub-nosed monkey), and Simias (pig-tailed langur) all belong to this subfamily.
Simian Virus 5
A species of RUBULAVIRUS originally isolated from cultured primary monkey cells. Its natural host is the DOG in which it causes kennel cough, but it can also infect humans.
Cercopithecus Aethiops
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannas of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Erythrocebus
a genus of Old World monkeys of the family CERCOPITHECINAE that inhabit the forests and savannas of Africa. It contains only one species, ERYTHROCEBUS PATAS, also known as the patas monkey or red monkey.
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