Humanised substitutes for animal sera in human mesenchymal stem cell culture and differentiation.
Summary of "Humanised substitutes for animal sera in human mesenchymal stem cell culture and differentiation."
The use of animal sera in cell culture is a major deterrent for using such cells in cellular therapy owing to potential contamination with xenogenic agents. This necessitates the development of 'humanized' supplements to overcome such problems. This study investigates the use of two 'human-derived' supplements, namely human platelet lysate (hPL) and umbilical cord blood serum (UCBS), in human mesenchymal stem cell (hMSCs) culture. Properties of hMSCs cultured in either of these supplements including growth kinetics and the differentiation potential were better than those cultured in animal sera-supplemented media. This is also the first report concerning hepatogenic differentiation of hMSCs cultured in UCBS-supplemented media. Development of 'humanized' alternatives to FBS could be revolutionary in terms of extrapolating results from in-vitro cultured / expanded hMSCs to direct clinical cellular therapeutic strategies.
Affiliation
Journal Details
This article was published in the following journal.
Name: Cell biology international
ISSN: 1095-8355
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21492095
- DOI: http://dx.doi.org/10.1042/CBI20100649
Medical and Biotech [MESH] Definitions
Bone Marrow Transplantation
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
Mesenchymal Stem Cell Transplantation
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
Mesenchymal Stem Cells
Cells that can develop into distinct mesenchymal tissue such as BONE; TENDONS; MUSCLES; ADIPOSE TISSUE; CARTILAGE; NERVE TISSUE; and BLOOD and BLOOD VESSELS.
Uukuniemi Virus
A species in the genus PHLEBOVIRUS of the family BUNYAVIRIDAE, infecting vertebrates and vectored by ticks. It has not been associated with human disease though antibodies have been isolated from human sera.
Nuclear Transfer Techniques
Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell. Often the nucleus of a somatic cell is transferred into a recipient OVUM or stem cell (STEM CELLS) with the nucleus removed. This technology may provide means to generate autologous diploid pluripotent cell for therapeutic cloning, and a model for studying NUCLEAR REPROGRAMMING in embryonic stem cells. Nuclear transfer was first accomplished with frog eggs (RANA PIPIENS) and reported in 1952.
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