A new subspecies of Anoxybacillus flavithermus subsp. yunnanensis subsp. nov. with very high ethanol tolerance.
Summary of "A new subspecies of Anoxybacillus flavithermus subsp. yunnanensis subsp. nov. with very high ethanol tolerance."
In a search for thermophilic ethanol-tolerant bacteria, water-sediment samples collected at springs in Yunnan province of China were screened by ethanol enrichment. A novel thermophilic bacterium, strain E13(T) , was isolated. It exhibits a unique and remarkable ability to preferably grow in the presence of ethanol and is able to tolerate 13% (v/v) ethanol at 60°C. The isolate is a facultative aerobic, Gram-positive, motile, spore-forming rod that is capable of utilizing a range of carbon sources, such as xylose, arabinose and cellobiose. Phylogenetic analysis based on 16S rRNA gene similarity showed the strain to be affiliated with the species Anoxybacillus flavithermus (99.2% sequence similarity). DNA-DNA hybridization comparisons demonstrated a 64.8% DNA-DNA relatedness between strain E13(T) and A. flavithermus DSM 2641(T) . On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel subspecies of A. flavithermus, for which the name Anoxybacillus flavithermus subsp. yunnanensis subsp. nov. is proposed. The type strain of this subspecies is E13(T) (=CCTCC AB2010187(T) =KCTC 13759(T) ).
Engineering Technology Research Center of Microorganisms and Biocatalysis, Anhui Province, School of Life Sciences, Anhui University, P.R. China Technical Center of Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei, P.R. China.
This article was published in the following journal.
Name: FEMS microbiology letters
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21521361
- DOI: http://dx.doi.org/10.1111/j.1574-6968.2011.02294.x
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