A Novel Animal Model for Hyperdynamic Airway Collapse.
Summary of "A Novel Animal Model for Hyperdynamic Airway Collapse."
ABSTRACTRationale: Tracheobronchomalacia (TBM) is increasingly recognized as a condition associated with significant pulmonary morbidity. However, treatment is invasive and complex, and since there is no appropriate animal model, novel diagnostic and treatment strategies are difficult to evaluate. We endeavored to develop a reliable airway model to simulate hyperdynamic airway collapse in humans.
METHODS:
Seven 20kg male sheep were enrolled in this study. Tracheomalacia was created by submucosal resection of more than 50% of the circumference of ten consecutive cervical tracheal cartilage rings through a midline cervical incision. A silicone stent was placed in the trachea to prevent airway collapse during recovery. Tracheal collapsibility was assessed at protocol specific time points by bronchoscopy and multi -detector computed tomography (MDCT) imaging while temporarily removing the stent. Esophageal pressure and flow data were collected to assess flow limitation during spontaneous breathing.
RESULTS:
All animals tolerated the surgical procedure well and were stented without complications. One sheep died at 2 weeks due to respiratory failure related to stent migration. In all sheep, near total forced inspiratory airway collapse was observed up to 3 months post-procedure. Esophageal manometry demonstrated flow limitation associated with large negative pleural pressure swings during rapid spontaneous inhalation.
CONCLUSIONS:
Hyperdynamic airway collapse can reliably be induced with this technique. It may serve as a model for evaluation of novel diagnostic and therapeutic strategies for TBM.
Affiliation
Chest Disease Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston MA, USA.
Journal Details
This article was published in the following journal.
Name: Chest
ISSN: 1931-3543
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20651025
- DOI: http://dx.doi.org/10.1378/chest.10-0165
Medical and Biotech [MESH] Definitions
Asthma
A form of bronchial disorder with three distinct components: airway hyper-responsiveness (RESPIRATORY HYPERSENSITIVITY), airway INFLAMMATION, and intermittent AIRWAY OBSTRUCTION. It is characterized by spasmodic contraction of airway smooth muscle, WHEEZING, and dyspnea (DYSPNEA, PAROXYSMAL).
Models, Statistical
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
Colony Collapse
The sudden collapse and disappearance or diminution of a colony of organisms.
Airway Remodeling
The structural changes in the number, mass, size and/or composition of the airway tissues.
Animal Use Alternatives
Alternatives to the use of animals in research, testing, and education. The alternatives may include reduction in the number of animals used, replacement of animals with a non-animal model or with animals of a species lower phylogenetically, or refinement of methods to minimize pain and distress of animals used.
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