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Placental Proteomics Reveal Insights into Fetal Alcohol Spectrum Disorders.

08:00 EDT 19th July 2017 | BioPortfolio

Summary of "Placental Proteomics Reveal Insights into Fetal Alcohol Spectrum Disorders."

Fetal alcohol spectrum disorders (FASD) describe many of the well-known neurodevelopmental deficits afflicting children exposed to alcohol in utero. The effects of alcohol on the maternal-fetal interface, especially the placenta, have been less explored. We herein hypothesized that chronic binge alcohol exposure during pregnancy significantly alters the placental protein profile in a rat FASD model
METHODS:
Pregnant rats were orogastrically treated daily with alcohol (4.5 g/kg, gestational day (GD) 5-10; 6.0 g/kg, GD 11-19) or 50% maltose dextrin (isocalorically matched pair-fed controls). On GD 20, placentae were collected, flash frozen, and stored until tissues were homogenized. Protein lysates were denatured, reduced, captured on a 10 kDa spin filter and digested. Peptides were eluted, reconstituted, and analyzed by a Q Exactive(™) Hybrid Quadrupole-Orbitrap(™) mass spectrometer
RESULTS:
Mass spectrometry analysis identified 2,285 placental proteins based on normalized spectral counts and 2000 proteins by intensity based absolute quantification. 45 placental proteins were significantly (P<0.05) altered by gestational alcohol exposure by both quantification approaches. These included proteins directly related to alcohol metabolism; specific isoforms of alcohol dehydrogenase and aldehyde dehydrogenase were upregulated in the alcohol group. Ingenuity analysis identified ethanol degradation as the most significantly altered canonical pathway in placenta, and fetal/organ development as most altered function, with increased risk for metabolic, neurological, and cardiovascular diseases. Physiologic roles of the significantly altered proteins were related to early pregnancy adaptations, implantation, gestational diseases, fetal organ development, neurodevelopment, and immune functions
CONCLUSIONS:
We conclude that the placenta is a valuable organ not only to understand FASD etiology but it may also serve as a diagnostic tool to identify novel biomarkers for detecting the outcome of fetal alcohol exposure. Placental mass spectrometry analysis can offer sophisticated insights into identifying alcohol metabolism-related enzymes and regulators of fetal development. This article is protected by copyright. All rights reserved.

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Journal Details

This article was published in the following journal.

Name: Alcoholism, clinical and experimental research
ISSN: 1530-0277
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