Beneficial effects of synthetic KL4-surfactant in experimental lung transplantation.

06:00 EDT 24th July 2010 | BioPortfolio

Summary of "Beneficial effects of synthetic KL4-surfactant in experimental lung transplantation."

The aim of this study was to investigate whether intratracheal administration of a new synthetic surfactant that includes the cationic, hydrophobic 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4), might be effective in reducing ischemia-reperfusion injury after lung transplantation. Single left lung transplantation was performed in Landrace pigs 22 hours post harvest. KL4-surfactant at a dose of 25 mg (2.5 mL)/kg total phospholipid was instilled at 37 degrees C to the donor left lung (n=8) prior to explantation. Saline (2.5; 37 degrees C) was instilled into the donor left lung of the untreated group (n=6). Lung function in recipients was measured during 2 hr of reperfusion. Recipient left lung bronchoalveolar lavage (BAL) provided native cytometric, inflammatory marker, and surfactant data. KL4-surfactant treatment recovered oxygen levels in the recipient blood (Pa,O2/FiO2 of 424+/-60 mm Hg vs. 263+/-101 mm Hg in untreated group; p=0.01) and normalized alveolar-arterial oxygen gradient. Surfactant biophysical function was also recovered in KL4-surfactant-treated lungs. This was associated with decreased C-reactive protein levels in BAL and recovery of surfactant protein A content, normalized protein/phospholipid ratios, and lower levels of both lipid peroxides and protein carbonyls in large surfactant aggregates. These findings suggest an important protective role for KL4-surfactant treatment in lung transplantation.


Complutense University and CIBERES (Respiratory Research Center) Madrid Spain.

Journal Details

This article was published in the following journal.

Name: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology
ISSN: 1399-3003


DeepDyve research library

PubMed Articles [30141 Associated PubMed Articles listed on BioPortfolio]

The Effects of Lung Protective Ventilation or Hypercapnic Acidosis on Gas Exchange and Lung Injury in Surfactant Deficient Rabbits.

Permissive hypercapnia has been shown to reduce lung injury in subjects with surfactant deficiency. Experimental studies suggest that hypercapnic acidosis by itself rather than decreased tidal volume ...

Effects of the New Generation Synthetic Reconstituted Surfactant CHF5633 on Pro- and Anti-Inflammatory Cytokine Expression in Native and LPS-Stimulated Adult CD14+ Monocytes.

Surfactant replacement therapy is the standard of care for the prevention and treatment of neonatal respiratory distress syndrome. New generation synthetic surfactants represent a promising alternativ...

Lung surfactant metabolism: early in life, early in disease and target in cell therapy.

Lung surfactant is a complex mixture of lipids and proteins lining the alveolar epithelium. At the air-liquid interface, surfactant lowers surface tension, avoiding alveolar collapse and reducing the ...

Lung Transplantation in Icelanders.

Lung transplantation is a treatment option for end-stage lung diseases, excluding lung cancer, when life expectancy is short and quality of life is poor. In most instances pulmonary function and quali...

Characterization of surfactant alterations in pigs infected with Actinobacillus pleuropneumoniae.

Purpose/Aim of the study: Surfactant, a surface active complex of phospholipids and proteins located at the inner surface of alveoli and small conducting airways is necessary for breathing. Bacterial ...

Clinical Trials [8676 Associated Clinical Trials listed on BioPortfolio]

The Use of Surfactant in Lung Transplantation: A Randomized Control Pilot Study

1. Working Hypothesis: The purpose of the trial is to study the effect of exogenous calf surfactant (calfactant) on the prevention of primary graft failure due to ischemic-reper...

Human Surfactant Treatment of Respiratory Distress Syndrome Bicenter Trial

To determine if surfactant administration at birth in infants at high risk for respiratory distress syndrome (RDS) modified the clinical course of the syndrome.

QT-Prolongation in Lung Transplantation

Experimental intervention: electrocardiography. Control intervention: none Duration of intervention per patient/subject: 5 min, observation 6 months Key inclusion criteria: ...

Edaravone on the Ischemia-Reperfusion(I/R) Injury in Kidney Transplantation Patients

Ischemia-reperfusion (I/R) injury is a prominent cause of delayed graft function(DGF) after kidney transplantation. Reactive oxygen species play a crucial role in I/R injury. Edaravone is ...

Does The Surfactant Administration by Aerosolization Effective?

The present study was designed to evaluate, in premature babies with RDS breathing spontaneously, the efficacy of combined treatment with nasal continuous positive airway pressure (CPAP) a...

Medical and Biotech [MESH] Definitions

An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens and enhances their opsinization and killing by phagocytic cells. Surfactant protein D contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.

An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens, resulting in their opsinization. It also stimulates MACROPHAGES to undergo PHAGOCYTOSIS of microorganisms. Surfactant protein A contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.

Experimental transplantation of neoplasms in laboratory animals for research purposes.

A pulmonary surfactant associated protein that plays a role in alveolar stability by lowering the surface tension at the air-liquid interface. It is a membrane-bound protein that constitutes 1-2% of the pulmonary surfactant mass. Pulmonary surfactant-associated protein C is one of the most hydrophobic peptides yet isolated and contains an alpha-helical domain with a central poly-valine segment that binds to phospholipid bilayers.

A pulmonary surfactant associated-protein that plays an essential role in alveolar stability by lowering the surface tension at the air-liquid interface. Inherited deficiency of pulmonary surfactant-associated protein B is one cause of RESPIRATORY DISTRESS SYNDROME, NEWBORN.

Quick Search

DeepDyve research library

Relevant Topics

Pulmonary relating to or associated with the lungs eg Asthma, chronic bronchitis, emphysema, COPD, Cystic Fibrosis, Influenza,  Lung Cancer, Pneumonia, Pulmonary Arterial Hypertension, Sleep Disorders etc Follow and track Lung Cancer News ...

Organ transplantation is the moving of an organ from one body to another or from a donor site to another location on the patient's own body, for the purpose of replacing the recipient's damaged or absent organ. The emerging field of regenerative ...

Searches Linking to this Article