Beneficial effects of synthetic KL4-surfactant in experimental lung transplantation.

21:57 EDT 26th March 2015 | BioPortfolio

Summary of "Beneficial effects of synthetic KL4-surfactant in experimental lung transplantation."

The aim of this study was to investigate whether intratracheal administration of a new synthetic surfactant that includes the cationic, hydrophobic 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4), might be effective in reducing ischemia-reperfusion injury after lung transplantation. Single left lung transplantation was performed in Landrace pigs 22 hours post harvest. KL4-surfactant at a dose of 25 mg (2.5 mL)/kg total phospholipid was instilled at 37 degrees C to the donor left lung (n=8) prior to explantation. Saline (2.5; 37 degrees C) was instilled into the donor left lung of the untreated group (n=6). Lung function in recipients was measured during 2 hr of reperfusion. Recipient left lung bronchoalveolar lavage (BAL) provided native cytometric, inflammatory marker, and surfactant data. KL4-surfactant treatment recovered oxygen levels in the recipient blood (Pa,O2/FiO2 of 424+/-60 mm Hg vs. 263+/-101 mm Hg in untreated group; p=0.01) and normalized alveolar-arterial oxygen gradient. Surfactant biophysical function was also recovered in KL4-surfactant-treated lungs. This was associated with decreased C-reactive protein levels in BAL and recovery of surfactant protein A content, normalized protein/phospholipid ratios, and lower levels of both lipid peroxides and protein carbonyls in large surfactant aggregates. These findings suggest an important protective role for KL4-surfactant treatment in lung transplantation.


Complutense University and CIBERES (Respiratory Research Center) Madrid Spain.

Journal Details

This article was published in the following journal.

Name: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology
ISSN: 1399-3003


PubMed Articles [27437 Associated PubMed Articles listed on BioPortfolio]

Avoiding the prenatal programming effects of glucocorticoids: are there alternative treatments for the induction of antenatal lung maturation?

Abstract Background: The long-term outcomes of antenatal glucocorticoids (GCs) vary between reports, and have generated controversy in terms of repeated and single-course events, causing irreversible ...

Human mesenchymal stromal cell lysates as a novel strategy to recover liver function.

Aim: It is unknown if the beneficial effects of mesenchymal stromal cells (MSC) transplantation into the liver are dependent on their anchorage and differentiation into hepatocytes or rather the resul...

Surfactant with and without bronchoalveolar lavage in an experimental model of meconium aspiration syndrome.

Abstract Objectives: To assess the effect of surfactant administration, preceded or not by bronchoalveolar lavage (BAL) with dilute surfactant, on pulmonary function in experimental severe meconium as...

Single-lung transplantation in the United States: What happens to the other lung?

This study assessed treatment patterns and examined organ utilization in the setting of single-lung transplantation (SLT).

Mesenchymal Stem Cells Overexpressing Angiotensin-Converting Enzyme 2 Rescue Lipopolysaccharide-Induced Lung Injury.

Bone marrow-derived mesenchymal stem cells (MSCs), which have beneficial effects in acute lung injury (ALI), can serve as a vehicle for gene therapy. Angiotensin converting enzyme 2 (ACE2), a counter-...

Clinical Trials [6033 Associated Clinical Trials listed on BioPortfolio]

The Use of Surfactant in Lung Transplantation: A Randomized Control Pilot Study

1. Working Hypothesis: The purpose of the trial is to study the effect of exogenous calf surfactant (calfactant) on the prevention of primary graft failure due to ischemic-reper...

Human Surfactant Treatment of Respiratory Distress Syndrome Bicenter Trial

To determine if surfactant administration at birth in infants at high risk for respiratory distress syndrome (RDS) modified the clinical course of the syndrome.

QT-Prolongation in Lung Transplantation

Experimental intervention: electrocardiography. Control intervention: none Duration of intervention per patient/subject: 5 min, observation 6 months Key inclusion criteria: ...

Impact of Surfactant's Availability on Newborns

To identify what happened to specific groups of newborns after surfactant was introduced to the market. Were the same benefits with regard to morbidity, mortality, and resource use in evid...

Surfactant Lavage vs. Bolus Surfactant in Neonates With Meconium Aspiration

The objective of this pilot study is to examine the feasibility and safety of performing a larger trial to assess outcomes following treatment of meconium aspiration syndrome with surfacta...

Medical and Biotech [MESH] Definitions

An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens and enhances their opsinization and killing by phagocytic cells. Surfactant protein D contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.

An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens, resulting in their opsinization. It also stimulates MACROPHAGES to undergo PHAGOCYTOSIS of microorganisms. Surfactant protein A contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.

Experimental transplantation of neoplasms in laboratory animals for research purposes.

A pulmonary surfactant associated protein that plays a role in alveolar stability by lowering the surface tension at the air-liquid interface. It is a membrane-bound protein that constitutes 1-2% of the pulmonary surfactant mass. Pulmonary surfactant-associated protein C is one of the most hydrophobic peptides yet isolated and contains an alpha-helical domain with a central poly-valine segment that binds to phospholipid bilayers.

A pulmonary surfactant associated-protein that plays an essential role in alveolar stability by lowering the surface tension at the air-liquid interface. Inherited deficiency of pulmonary surfactant-associated protein B is one cause of RESPIRATORY DISTRESS SYNDROME, NEWBORN.

Search BioPortfolio:

Relevant Topics

Latest News Clinical Trials Research Drugs Reports Corporate
Pulmonary relating to or associated with the lungs eg Asthma, chronic bronchitis, emphysema, COPD, Cystic Fibrosis, Influenza,  Lung Cancer, Pneumonia, Pulmonary Arterial Hypertension, Sleep Disorders etc Follow and track Lung Cancer News ...

Latest News Clinical Trials Research Drugs Reports Corporate
Organ transplantation is the moving of an organ from one body to another or from a donor site to another location on the patient's own body, for the purpose of replacing the recipient's damaged or absent organ. The emerging field of regenerative ...


Searches Linking to this Article