Carbon nanospheres for highly sensitive electrochemical detection of sequence-specific protein-DNA interactions.
Summary of "Carbon nanospheres for highly sensitive electrochemical detection of sequence-specific protein-DNA interactions."
A carbon nanosphere-based electrochemical detection method is developed for the highly sensitive detection of Sp1-DNA interactions.
Affiliation
Institute of Functional Nano & Soft Materials and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou, Jiangsu 215123, China. yangl@suda.edu.cn, rpeng@suda.edu.cn zhkang@suda.edu.cn.
Journal Details
This article was published in the following journal.
Name: Chemical communications (Cambridge, England)
ISSN: 1364-548X
Pages: 8316-8
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21660335
- DOI: http://dx.doi.org/10.1039/c1cc12375k
Medical and Biotech [MESH] Definitions
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Limulus Test
Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.
Electrophoresis, Capillary
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
Papillomaviridae
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology.
Dna Sequence, Unstable
A region of DNA that is highly polymorphic and is prone to strand breaks, rearrangements or other MUTATIONS because of the nature of its sequence. These regions often harbor palindromic, or repetitive sequences (REPETITIVE SEQUENCES, NUCLEIC ACID). Variability in stability of the DNA sequence is seen at CHROMOSOME FRAGILE SITES.
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